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首页> 外文期刊>Journal of Experimental Botany >The beta-ketoacyl-CoA synthase KCS13 regulates the cold response in cotton by modulating lipid and oxylipin biosynthesis
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The beta-ketoacyl-CoA synthase KCS13 regulates the cold response in cotton by modulating lipid and oxylipin biosynthesis

机译：β-酮酰基-CoA合酶KCS13通过调节脂质和奥氧脂生物合成来调节棉花的冷应激

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摘要

Cold stress is a key environmental factor that affects plant development and productivity. In this study, RNA-seq in cotton following cold-stress treatment resulted in the identification of 5239 differentially expressed genes (DEGs) between two cultivars with differing sensitivity to low temperatures, among which GhKCS13 was found to be involved in the response. Transgenic plants overexpressing GhKCS13 showed increased sensitivity to cold stress. KEGG analysis of 418 DEGs in both GhKCS13-overexpressing and RNAi lines after treatment at 4 degrees C indicated that lipid biosynthesis and linoleic acid metabolism were related to cold stress. ESI-MS/MS analysis showed that overexpression of GhKCS13 led to modifications in the composition of sphingolipids and glycerolipids in the leaves, which might alter the fluidity of the cell membrane under cold conditions. In particular, differences in levels of jasmonic acid (JA) in GhKCS13 transgenic lines suggested that, together with lysophospholipids, it might mediate the cold-stress response. Our results suggest that overexpression of GhKCS13 probably causes remodeling of lipids in the endoplasmic reticulum and biosynthesis of lipid-derived JA in chloroplasts, which might account for the increased sensitivity to cold stress in the transgenic plants. Complex interactions between lipid components, lipid signaling molecules, and JA appear to determine the response to cold stress in cotton.
机译：冷应力是影响植物开发和生产力的关键环境因素。在该研究中，冷应激处理后RNA-SEQ在棉质之后，导致两种品种与低温敏感性不同的差异表达的基因（DEG）鉴定，发现GHKCS13参与响应。过表达GHKCS13的转基因植物表明对冷应激的敏感性增加。在4摄氏度处理后GHKC13过度抑制和RNAi线路中的418次Degs分析表明脂质生物合成和亚油酸代谢与冷应激有关。 ESI-MS / MS分析表明，GHKC13的过度表达导致叶片中的鞘脂和甘油脂的组成改性，这​​可能在冷条件下改变细胞膜的流动性。特别地，GHKCS13转基因系中茉莉酸（JA）水平的差异表明，与溶血磷脂一起，它可以介导冷应激反应。我们的研究结果表明，GHKCS13的过度表达可能导致脂质网状物中的脂质的重塑，并且在叶绿体中的脂质衍生的JA的生物合成，这可能考虑转基因植物中对冷应激的敏感性增加。脂质组分，脂质信号分子和JA之间的复杂相互作用似乎确定对棉花的冷应激的反应。

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  • 来源
    《Journal of Experimental Botany》 |2020年第18期|共16页
  • 作者

    Wang Qiongshan; Du Xueqiong; Zhou Ying; Xie Lijuan; Bie Shu; Tu Lili; Zhang Na; Yang Xiyan; Xiao Shi; Zhang Xianlong;

  • 作者单位

    Hubei Acad Agr Sci Econ Crop Res Inst Wuhan Hubei Peoples R China;

    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan Hubei Peoples R China;

    Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol &

    Guangdong Prov Key Lab Guangzhou Peoples R China;

    Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol &

    Guangdong Prov Key Lab Guangzhou Peoples R China;

    Hubei Acad Agr Sci Econ Crop Res Inst Wuhan Hubei Peoples R China;

    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan Hubei Peoples R China;

    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan Hubei Peoples R China;

    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan Hubei Peoples R China;

    Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol &

    Guangdong Prov Key Lab Guangzhou Peoples R China;

    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan Hubei Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    beta-ketoacyl-CoA synthase; cold stress; cotton; Gossypium hirsutum; jasmonic acid; lipid; RNA-seq;

    机译：β-酮酰基-CoA合成酶;冷应激;棉花;Gossypium hirsutum;茉莉酸;脂质;RNA-SEQ;

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