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首页> 外文期刊>Journal of Experimental Botany >Variability in the redox status of plant 2-Cys peroxiredoxins in relation to species and light cycle
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Variability in the redox status of plant 2-Cys peroxiredoxins in relation to species and light cycle

机译：植物2-Cys过氧毒毒素的氧化还原状态的变异性与物种和光周期相关

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摘要

Plant 2-Cys peroxiredoxins (2-CysPRXs) are abundant plastidial thiol-peroxidases involved in key signaling processes such as photosynthesis deactivation at night. Their functions rely on the redox status of their two cysteines and on the enzyme quaternary structure, knowledge of which remains poor in plant cells. Using ex vivo and biochemical approaches, we thoroughly characterized the 2-CysPRX dimer/monomer distribution, hyperoxidation level, and thiol content in Arabidopsis, barley, and potato in relation to the light cycle. Our data reveal that the enzyme hyperoxidization level and its distribution as a dimer and monomer vary through the light cycle in a species-dependent manner. A differential susceptibility to hyperoxidation was observed for the two Arabidopsis 2-CysPRX isoforms and among the proteins of the three species, and was associated to sequence variation in hyperoxidation resistance motifs. Alkylation experiments indicate that only a minor fraction of the 2-CysPRX pool carries one free thiol in the three species, and that this content does not change during the light period. We conclude that most plastidial 2-CysPRX forms are oxidized and propose that there is a species-dependent variability in their functions since dimer and hyperoxidized forms fulfill distinct roles regarding direct oxidation of partners and signal transmission.
机译：植物2-Cys过氧化毒素（2-Cysprxs）是丰富的塑性化硫醇 - 过氧化物酶，涉及夜间光合作用的关键信号传导过程，如夜间光合作用。它们的功能依赖于其两种半胱氨酸的氧化还原状态以及酶四季结构，其知识在植物细胞中仍然较差。使用前体内和生化方法，我们彻底地表征了拟南芥，大麦和马铃薯的2-Cysprx二聚体/单体分布，升高水平和硫醇含量。我们的数据表明，酶高氧化水平及其作为二聚体和单体的分布以依赖性方式的光循环变化。对于两种拟南芥2-CYSPRX同种型以及三种物种的蛋白质，观察到对升高氧化的差异敏感性，并且与升高氧化抗性基序列的序列变异相关。烷基化实验表明，仅在三种物种中只有一个小部分的2-CySprx池携带一种游离硫醇，并且在光时段期间这种内容不会改变。我们得出结论，大多数塑性2-CySpRX形式被氧化并提出其功能中存在依赖性可变性，因为二聚体和超氧化形式满足了关于合作伙伴的直接氧化和信号传输的不同作用。

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