...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >The DEAD-box RNA helicase SHI2 functions in repression of salt-inducible genes and regulation of cold-inducible gene splicing
【24h】

The DEAD-box RNA helicase SHI2 functions in repression of salt-inducible genes and regulation of cold-inducible gene splicing

机译：死箱RNA螺旋酶SHI2用于抑制盐诱导基因的作用和冷诱导基因剪接调控

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gene regulation is central for growth, development, and adaptation to environmental changes in all living organisms. Many genes are induced by environmental cues, and the expression of these inducible genes is often repressed under normal conditions. Here, we show that the SHINY2 (SHI2) gene is important for repressing salt-inducible genes and also plays a role in cold response. The shi2 mutant displayed hypersensitivity to cold, abscisic acid (ABA), and LiCl. Map-based cloning demonstrates that SHI2 encodes a DEAD- (Asp-Glu-Ala-Asp) box RNA helicase with similarity to a yeast splicing factor. Transcriptomic analysis of the shi2 mutant in response to cold revealed that the shi2 mutation decreased the number of cold-responsive genes and the magnitude of their response, and resulted in the mis-splicing of some cold-responsive genes. Under salt stress, however, the shi2 mutation increased the number of salt-responsive genes but had a negligible effect on mRNA splicing. Our results suggest that SHI2 is a component in a ready-for-transcription repressor complex important for gene repression under normal conditions, and for gene activation and transcription under stress conditions. In addition, SHI2 also serves as a splicing factor required for proper splicing of cold-responsive genes and affects 5' capping and polyadenylation site selection.
机译：基因调节是生长，发展和适应所有生物体的环境变化的核心。由环境提示诱导许多基因，并且在正常条件下通常压抑这些诱导基因的表达。在这里，我们表明Shiny2（Shi2）基因对于抑制盐诱导基因很重要，并且在寒冷反应中也发挥作用。 SHI2突变体显示出冷，脱钙（ABA）和LICL的过敏率。基于地图的克隆表明SHI2编码了与酵母剪接因子相似的死（ASP-Glu-ALA-ASP）盒RNA螺旋酶。对Shi2突变体的转录组分析响应于寒冷显示SHI2突变降低了冷响应基因的数量和响应的大小，并导致一些冷响应基因的错误剪接。然而，在盐胁迫下，SHI2突变增加了盐响应基因的数量，但对mRNA剪接具有可忽略的影响。我们的研究结果表明，Shi2是在正常条件下的基因抑制和基因抑制和基因活化和转录在应激条件下的重要转换抑制体复合体中的组成部分。此外，SHI2还用作正确拼接冷响应基因所需的剪接因子，并影响5'封端和多腺苷酸化位点选择。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号