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首页> 外文期刊>Journal of Experimental Botany >Comparative studies of C-3 and C-4 Atriplex hybrids in the genomics era: physiological assessments
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Comparative studies of C-3 and C-4 Atriplex hybrids in the genomics era: physiological assessments

机译：基因组学时代C-3和C-4阿氏杂交种的比较研究：生理评估

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We crossed the C-3 species Atriplex prostrata with the C-4 species Atriplex rosea to produce F-1 and F-2 hybrids. All hybrids exhibited C-3-like delta C-13 values, and had reduced rates of net CO2 assimilation compared with A. prostrata. The activities of the major C-4 cycle enzymes PEP carboxylase, NAD-malic enzyme, and pyruvate-P-i dikinase in the hybrids were at most 36% of the C-4 values. These results demonstrate the C-4 metabolic cycle was disrupted in the hybrids. Photosynthetic CO2 compensation points (.) of the hybrids were generally midway between the C-3 and C-4 values, and in most hybrids were accompanied by low, C-3-like activities in one or more of the major C-4 cycle enzymes. This supports the possibility that most hybrids use a photorespiratory glycine shuttle to concentrate CO2 into the bundle sheath cells. One hybrid exhibited a C-4-like Gamma of 4 mu mol mol(-1), indicating engagement of a C-4 metabolic cycle. Consistently, this hybrid had elevated activities of all measured C-4 cycle enzymes relative to the C-3 parent; however, C3-like carbon isotope ratios indicate the low. is mainly due to a photorespiratory glycine shuttle. The anatomy of the hybrids resembled that of C-3-C-4 intermediate species using a glycine shuttle to concentrate CO2 in the bundle sheath, and is further evidence that this physiology is the predominant, default condition of the F-2 hybrids. Progeny of these hybrids should further segregate C-3 and C-4 traits and in doing so assist in the discovery of C-4 genes using high-throughput methods of the genomics era.
机译：我们用C-4物种AtripleS rosea越过C-3物种Atriplex Prostata，以生产F-1和F-2杂种。所有杂种都表现出C-3样Delta C-13值，与A. Prostrata相比，净二氧化碳同化率降低。杂交种中的主要C-4循环酶Pep羧化酶，NAD-母酶和丙酮酸-P-I直立通酶的活性为C-4值的36％。这些结果证明了C-4代谢循环在杂种中断。杂种的光合作用CO2补偿点（。）通常在C-3和C-4值之间中途，并且在大多数杂种中伴有一个或多个主要的C-4循环中的低，C-3样活动酶。这支持大多数杂交种使用光致血红素梭子将CO2集中到束鞘细胞中的可能性。一种杂种显示出4μmolmol（-1）的C-4样γ，表明C-4代谢循环的接合。始终如一地，该杂种相对于C-3父母的所有测量的C-4循环酶的活性升高;然而，C3样碳同位素比率表明低。主要是由于光致甘氨酸梭梭。杂种的解剖学类似于C-3-C-4中间物种的使用甘氨酸梭中以浓缩CO 2在束鞘中浓缩CO 2，并且进一步证据表明这种生理学是F-2杂种的主要默认条件。这些杂种的后代应该进一步分离C-3和C-4特征，并且在使用基因组学时代的高通量方法发现C-4基因的协助。

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