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首页> 外文期刊>Journal of Experimental Botany >Transcriptome analysis reveals novel enzymes for apo-carotenoid biosynthesis in saffron and allows construction of a pathway for crocetin synthesis in yeast
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Transcriptome analysis reveals novel enzymes for apo-carotenoid biosynthesis in saffron and allows construction of a pathway for crocetin synthesis in yeast

机译：转录组分析揭示了番红花中Apo类胡萝卜素生物合成的新酶，并允许在酵母中构建鳄鱼合成的途径

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Crocus sativus is generally considered the source of saffron spice which is rich in apo-carotenoid compounds such as crocins, crocetin, picrocrocin, and safranal, which possess effective pharmacological activities. However, little is known about the exact genes involved in apo-carotenoid biosynthesis in saffron and the potential mechanism of specific accumulation in the stigma. In this study, we integrated stigmas at different developmental stages to perform in-depth transcriptome and dynamic metabolomic analyses to discover the potential key catalytic steps involved in apo-carotenoid biosynthesis in saffron. A total of 61 202 unigenes were obtained, and 28 regulators and 32 putative carotenogenic genes were captured after the co-expression network analysis. Moreover, 15 candidate genes were predicted to be closely related to safranal and crocin production, in which one aldehyde dehydrogenase (CsALDH3) was validated to oxidize crocetin dialdehyde into crocetin and a crocetin-producing yeast strain was created. In addition, a new branch pathway that catalyses the conversion of geranyl-geranyl pyrophosphate to copalol and ent-kaurene by the class II diterpene synthase CsCPS1 and three class I diterpene synthases CsEKL1/2/3 were investigated for the first time. Such gene to apo-carotenoid landscapes illuminate the synthetic charactersistics and regulators of apo-carotenoid biosynthesis, laying the foundation for a deep understanding of the biosynthesis mechanism and metabolic engineering of apo-carotenoids in plants or microbes.
机译：番红花Sativus通常被认为是藏红花素香料的来源，其富含Apo类动物骨化合物，如鳄鱼，鳄鱼，Picrocrocin和Safranal，其具有有效的药理学活性。然而，关于藏红花中Apo类胡萝卜素生物合成的确切基因少知之甚少，以及耻辱中的特异性积累的潜在机制。在这项研究中，我们在不同发育阶段的颗粒综合，以进行深入的转录组和动态代谢分析，以发现藏红花中Apo类动物骨化生物合成中涉及的潜在关键催化步骤。在共表达网络分析之后，获得了总共6122个unigenes，并且在共表达网络分析后捕获了28个调节剂和32个调用的致癌基因。此外，预计将15个候选基因与番红豆和羊角生成密切相关，其中验证了一种醛脱氢酶（CSALDH3）以将番醇蛋白二醛氧化成番红豆素，产生番腺苷产生番腺苷酸酵母菌株。此外，首次研究了催化催化天竺葵 - 大烷基与焦酚和甾醇的转化为二硝基合酶CSCPS1和三类I类二萜合酶CSEKL1 / 2/3的新分支途径。这种基因对Apo-cotenoid景观照亮了Apo-cottenoid生物合成的合成性状和调节因子，奠定了深入了解植物或微生物在植物或微生物中Apo-cotootenoids的生物合成机制和代谢工程的基础。

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