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首页> 外文期刊>Journal of Experimental Botany >Flavonoids are indispensable for complete male fertility in rice
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Flavonoids are indispensable for complete male fertility in rice

机译：黄酮类化合物对于水稻中的完全男性生育是必不可少的

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Flavonoids are essential for male fertility in some but not all plant species. In rice (Oryza sativa), the chalcone synthase mutant oschs1 produces flavonoid-depleted pollen and is male sterile. The mutant pollen grains are viable with normal structure, but they display reduced germination rate and pollen-tube length. Analysis of oschs1/+ heterozygous lines shows that pollen flavonoid deposition is a paternal effect and fertility is independent of the haploid genotypes (OsCHS1 or oschs1). To understand which classes of flavonoids are involved in male fertility, we conducted detailed analysis of rice mutants for branch-point enzymes of the downstream flavonoid pathways, including flavanone 3-hydroxylase (OsF3H; flavonol pathway entry enzyme), flavone synthase II (CYP93G1; flavone pathway entry enzyme), and flavanone 2-hydroxylase (CYP93G2; flavone C- glycoside pathway entry enzyme). Rice osf3h and cyp93g1 cyp93g2 CRISPR/Cas9 mutants, and cyp93g1 and cyp93g2 T-DNA insertion mutants showed altered flavonoid profiles in anthers, but only the osf3h and cyp93g1 cyp93g2 mutants displayed reduction in seed yield. Our findings indicate that flavonoids are essential for complete male fertility in rice and a combination of different classes (flavanones, flavonols, flavones, and flavone C-glycosides) appears to be important, as opposed to the essential role played primarily by flavonols that has been previously reported in several plant species.
机译：黄酮类化合物对于一些但不是所有植物物种的男性生育是必不可少的。在水稻（Oryza sativa）中，Chalcone合成酶突变体OSCHS1产生黄酮类耗尽的花粉，是雄性无菌。突变花粉晶粒具有正常结构可行，但它们显示出降低的发芽率和花粉管长度。对OSCHS1 / +杂合线的分析表明，花粉黄酮沉积是父系效应，肥力与单倍体基因型（OSCHS1或OSCHS1）无关。为了了解哪些类类黄酮类化合物参与男性生育率，我们对下游黄酮途径的分支点酶进行了详细的分析，包括黄酮3-羟化酶（OSF3H;黄酮醇途径进入酶，黄酮合成酶II（CYP93G1;黄酮途径进入酶，香料2-羟化酶（CYP93G2;黄酮C-糖苷途径进入酶）。水稻OSF3H和CYP93G1 CYP93G2 CRISPR / CAS9突变体和CYP93G1和CYP93G2 T-DNA插入突变体在花药中显示出改变的黄酮类化谱，但只有OSF3H和CYP93G1 CYP93G2突变体的种子产量降低。我们的研究结果表明，黄酮类化合物对于水稻中完全雄性生育能力至关重要，不同类别（黄酮酮，黄酮酚，黄酮和黄酮C-糖苷）的组合似乎是重要的，而不是主要作用的基本作用以前报道了几种植物物种。

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