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首页> 外文期刊>Journal of Experimental Botany >PLATZ2 negatively regulates salt tolerance in Arabidopsis seedlings by directly suppressing the expression of the CBL4/SOS3 and CBL10/SCaBP8 genes
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PLATZ2 negatively regulates salt tolerance in Arabidopsis seedlings by directly suppressing the expression of the CBL4/SOS3 and CBL10/SCaBP8 genes

机译：Platz2通过直接抑制CBL4 / SOS3和CBL10 / SCABP8基因的表达，对拟南芥幼苗进行耐受耐盐性

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摘要

Although the salt overly sensitive (SOS) pathway plays essential roles in conferring salt tolerance in Arabidopsis thaliana, the regulatory mechanism underlying SOS gene expression remains largely unclear. In this study, AtPLATZ2 was found to function as a direct transcriptional suppressor of CBL4/SOS3 and CBL10/SCaBP8 in the Arabidopsis salt stress response. Compared with wild-type plants, transgenic plants constitutively overexpressing AtPLATZ2 exhibited increased sensitivity to salt stress. Loss of function of PLATZ2 had no observed salt stress phenotype in Arabidopsis, while the double mutant of PLATZ2 and PLATZ7 led to weaker salt stress tolerance than wild-type plants. Overexpression of AtPLATZ2 in transgenic plants decreased the expression of CBL4/SOS3 and CBL10/SCaBP8 under both normal and saline conditions. AtPLATZ2 directly bound to A/T-rich sequences in the CBL4/SOS3 and CBL10/SCaBP8 promoters in vitro and in vivo, and inhibited CBL4/SOS3 promoter activity in the plant leaves. The salt sensitivity of #11 plants constitutively overexpressing AtPLATZ2 was restored by the overexpression of CBL4/SOS3 and CBL10/SCaBP8. Salt stress-induced Na+ accumulation in both the shoots and roots was more exaggerated in AtPLATZ2-overexpressing plants than in the wild type. The salt stress-induced Na+ accumulation in #11 seedlings was also rescued by the overexpression of CBL4/SOS3 and CBL10/SCaBP8. Furthermore, the transcription of AtPLATZ2 was induced in response to salt stress. Collectively, these results suggest that AtPLATZ2 suppresses plant salt tolerance by directly inhibiting CBL4/SOS3 and CBL10/SCaBP8, and functions redundantly with PLATZ7.
机译：虽然盐过敏（SOS）途径在拟南芥中赋予耐盐性，但SOS基因表达的监管机制仍然很目的不清楚。在该研究中，发现ATPLATZ2在拟南芥盐应激反应中用作CBL4 / SOS3和CBL10 / SCABP8的直接转录抑制剂。与野生型植物相比，组成型过表达ATPLATZ2的转基因植物表现出对盐胁迫的敏感性增加。 Platz2的功能丧失在拟南芥中没有观察到的盐胁迫表型，而平板2和平板7的双突变体导致含盐胁迫耐受性而不是野生型植物。转基因植物中的ATPLATZ2的过度表达在正常和盐水条件下降低了CBL4 / SOS3和CBL10 / SCABP8的表达。 ATPLATZ2在体外和体内直接与CBL4 / SOS3和CBL10 / SCABP8启动子中的A / T-序列结合，并抑制植物叶中的CBL4 / SOS3启动子活性。通过CBL4 / SOS3和CBL10 / SCABP8的过度表达恢复组成患有过表达ATPLATZ2的＃11植物的盐敏感性。在Atplatz2过表达植物中比野生型盐胁迫诱导的盐和根部的积累更夸张。通过CBL4 / SOS3和CBL10 / SCABP8的过表达，还抵抗了＃11幼苗中的盐应激诱导的Na +积累。此外，响应于盐胁迫而诱导ATPLATZ2的转录。总的来说，这些结果表明ATPLATZ2通过直接抑制CBL4 / SOS3和CBL10 / SCABP8来抑制植物耐药性，并用PLATZ7冗余的功能。

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  • 来源
    《Journal of Experimental Botany》 |2020年第18期|共14页
  • 作者

    Liu Shasha; Yang Rui; Liu Miao; Zhang Shizhong; Yan Kang; Yang Guodong; Huang Jinguang; Zheng Chengchao; Wu Changai;

  • 作者单位

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    AtPLATZ2; A/T-rich sequences; CBL10/SCaBP8; CBL4/SOS3; salt overly sensitive (SOS); salt tolerance; transcriptional repressor;

    机译：Atplatz2;富含A / T的序列;CBL10 / SCABP8;CBL4 / SOS3;盐过度敏感（SOS）;耐盐性;转录压缩机;

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