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首页> 外文期刊>Journal of Experimental Botany >HvHOX9, a novel homeobox leucine zipper transcription factor, positively regulates aluminum tolerance in Tibetan wild barley
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HvHOX9, a novel homeobox leucine zipper transcription factor, positively regulates aluminum tolerance in Tibetan wild barley

机译：HVHOX9，一种新的Homeobox亮氨酸拉链转录因子，积极调节藏野大麦的铝耐受性

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摘要

Aluminum (Al) toxicity is the primary limiting factor of crop production on acid soils. Tibetan wild barley germplasm is a valuable source of potential genes for breeding barley with acid and Al tolerance. We performed microRNA and RNA sequencing using wild (XZ16, Al-tolerant; XZ61, Al-sensitive) and cultivated (Dayton, Al-tolerant) barley. A novel homeobox-leucine zipper transcription factor, HvHOX9, was identified as a target gene of miR166b and functionally characterized. HvHOX9 was up-regulated by Al stress in XZ16 (but unchanged in XZ61 and Dayton) and was significantly induced only in root tip. Phylogenetic analysis showed that HvHOX9 is most closely related to wheat TaHOX9 and orthologues of HvHOX9 are present in the closest algal relatives of Zygnematophyceae. Barley stripe mosaic virus-induced gene silencing of HvHOX9 in XZ16 led to significantly increased Al sensitivity but did not affect its sensitivity to other metals and low pH. Disruption of HvHOX9 did not change Al concentration in the root cell sap, but led to more Al accumulation in root cell wall after Al exposure. Silencing of HvHOX9 decreased H+ influx after Al exposure. Our findings suggest that miR166b/HvHOX9 play a critical role in Al tolerance by decreasing root cell wall Al binding and increasing apoplastic pH for Al detoxification in the root.
机译：铝（Al）毒性是酸性土壤作物生产的主要限制因素。西藏野生大麦种质是育种大麦的潜在基因的宝贵来源，酸和耐受性。我们使用野生（XZ16，耐抗体; XZ61，Al敏感）和栽培（Dayton，Al-Portorant）大麦进行MicroRNA和RNA测序。新的Homeobox-亮氨酸拉链转录因子，HVHOX9被鉴定为miR166b的靶基因，并在功能表征中。 HVHOX9通过XZ16中的Al胁迫上调（但在XZ61和Dayton中不变），仅在根尖中显着诱导。系统发育分析表明，HVHOX9与小麦Tahox9最密切相关，HVHOX9的矫形症中最近的ZygnemaTophyceae的藻类亲属存在。大麦条纹马赛克病毒诱导的XZ16中HVHOX9的基因沉默导致敏感性显着增加，但不会影响其对其他金属和低pH的敏感性。 HVHOX9的破坏在根细胞SAP中没有改变Al浓度，而是在Al接触后导致Root Cell壁中的更多Al积累。 HVHOX9的沉默在接触后降低了H +流入。我们的研究结果表明，MiR166B / HVHOX9通过减少根部细胞壁Al结合并增加根部中的Al解毒剂的妊娠率pH来发挥重要作用。

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