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首页> 外文期刊>Journal of Experimental Botany >A SOC1-like gene MtSOC1a promotes flowering and primary stem elongation in Medicago
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A SOC1-like gene MtSOC1a promotes flowering and primary stem elongation in Medicago

机译:SOC1样Gene MTSoc1A促进Medicago的开花和初级茎伸长率

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Medicago flowering, like that of Arabidopsis, is promoted by vernalization and long days, but alternative mechanisms are predicted because Medicago lacks the key regulators CO and FLC. Three Medicago SOC1-like genes, including MtSOC1a, were previously implicated in flowering control, but no legume soci mutants with altered flowering were reported. Here, reverse transciption-quantitative PCR (RT-qPCR) indicated that the timing and magnitude of MtSOC1a expression was regulated by the flowering promoter FTa1, while in situ hybridization indicated that MtSOC1a expression increased in the shoot apical meristem during the floral transition. A Mtsoc1a mutant showed delayed flowering and short primary stems. Overexpression of MtSOC1a partially rescued the flowering of Mtsoc1a, but caused a dramatic increase in primary stem height, well before the transition to flowering. Internode cell length correlated with stem height, indicating that MtSOC1a promotes cell elongation in the primary stem. However, application of gibberellin (GA(3)) caused stem elongation in both the wild type and Mtsoc1a, indicating that the mutant was not defective in gibberellin responsiveness. These results indicate that MtSOC1a may function as a floral integrator gene and promotes primary stem elongation. Overall, this study suggests that apart from some conservation with the Arabidopsis flowering network, MtSOC1a has a novel role in regulating aspects of shoot architecture.
机译:像拟南芥一样的Medicago开花是由春化的和漫长的日子促进的,但预测替代机制是因为Medicago缺乏关键调节器CO和FLC。三种Medicago Soc1样基因,包括MTSoc1a,先前涉及开花控制,但没有报道具有改变开花的豆科Soci突变体。这里,反向转发定量PCR(RT-QPCR)表明MTSoc1a表达的时序和大小由开花启动子FTA1调节,而原位杂交表明,在花转换期间芽顶部分类中的MTSoc1a表达增加。 MTSOC1A突变体显示出延迟开花和短发茎。 MTSOC1A的过度表达部分拯救了MTSOC1A的开花,但在过渡到开花之前,初级茎高的剧烈增加。节间电池长度与茎高相关,表明MTSoc1A促进初级茎中的细胞伸长。然而,在野生型和mTSoc1a中施用胃蛋白酶(Ga(3)),表明突变体在胃肠杆菌素反应性中没有缺陷。这些结果表明,MTSoc1a可以用作花卉积分剂基因并促进初级干延伸。总体而言,本研究表明,除了拟南芥开花网络的一些保护之外,MTSOC1A在调节射击建筑方面具有新的作用。

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