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首页> 外文期刊>Journal of Experimental Botany >N-glycosylation is involved in stomatal development by modulating the release of active abscisic acid and auxin in Arabidopsis
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N-glycosylation is involved in stomatal development by modulating the release of active abscisic acid and auxin in Arabidopsis

机译：通过调节拟南芥中的活性脱落酸和生长素的释放，N-糖基化参与气孔发育

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摘要

Asparagine-linked glycosylation (N-glycosylation) is one of the most important protein modifications in eukaryotes, affecting the folding, transport, and function of a wide range of proteins. However, little is known about the roles of N-glycosylation in the development of stomata in plants. In the present study, we provide evidence that the Arabidopsis stt3a-2 mutant, defective in oligosaccharyltransferase catalytic subunit STT3, has a greater transpirational water loss and weaker drought avoidance, accompanied by aberrant stomatal distribution. Through physiological, biochemical, and genetic analyses, we found that the abnormal stomatal density of stt3a-2 was partially attributed to low endogenous abscisic acid (ABA) and auxin (IAA) content. Exogenous application of ABA or IAA could partially rescue the mutant's salt-sensitive and abnormal stomatal phenotype. Further analyses revealed that the decrease of IAA or ABA in stt3a-2 seedlings was associated with the underglycosylation of beta-glucosidase (AtBG1), catalysing the conversion of conjugated ABA/IAA to active hormone. Our results provide strong evidence that N-glycosylation is involved in stomatal development and participates in abiotic stress tolerance by modulating the release of active plant hormones.
机译：天冬酰胺连接的糖基化（N-糖基化）是真核生物中最重要的蛋白质修饰之一，影响各种蛋白质的折叠，运输和功能。然而，关于N-糖基化在植物气孔发展中的作用知之甚少。在本研究中，我们提供了证据表明，在寡核酸脱硫酶催化亚单位STT3中有缺陷的拟南芥STT3A-2突变体具有更大的透析水分损失和较弱的干旱避免，伴随着异常的气孔分布。通过生理，生化和遗传分析，我们发现STT3A-2的异常气孔密度部分归因于低内源性脱离酸（ABA）和养蛋白（IAA）含量。 ABA或IAA的外源性应用可以部分拯救突变体的盐敏感和异常的气孔表型。进一步分析显示，STT3A-2幼苗中IAA或ABA的降低与β-葡糖苷酶（ATBG1）的底糖基化有关，催化缀合的ABA / IAA至活性激素的转化。我们的结果提供了强有力的证据，即N-糖基化参与气孔发育并通过调节活性植物激素的释放来参与非生物胁迫耐受性。

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  • 来源
    《Journal of Experimental Botany》 |2020年第19期|共15页
  • 作者

    Jiao Qingsong; Chen Tianshu; Niu Guanting; Zhang Huchen; Zhou ChangFang; Hong Zhi;

  • 作者单位

    Nanjing Univ Sch Life Sci NJU Adv Inst Life Sci NAILS State Key Lab Pharmaceut Biotechnol Nanjing 210046 Peoples R China;

    Nanjing Univ Sch Life Sci NJU Adv Inst Life Sci NAILS State Key Lab Pharmaceut Biotechnol Nanjing 210046 Peoples R China;

    Nanjing Univ Sch Life Sci NJU Adv Inst Life Sci NAILS State Key Lab Pharmaceut Biotechnol Nanjing 210046 Peoples R China;

    Nanjing Univ Sch Life Sci NJU Adv Inst Life Sci NAILS State Key Lab Pharmaceut Biotechnol Nanjing 210046 Peoples R China;

    Nanjing Univ Sch Life Sci NJU Adv Inst Life Sci NAILS State Key Lab Pharmaceut Biotechnol Nanjing 210046 Peoples R China;

    Nanjing Univ Sch Life Sci NJU Adv Inst Life Sci NAILS State Key Lab Pharmaceut Biotechnol Nanjing 210046 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    Abscisic acid; auxin; beta-glucosidase; N-glycosylation; stomatal development; stress tolerance;

    机译：脱落酸;植物蛋白;β-葡糖苷酶;N-糖基化;气孔发育;应力耐受性;

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