Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum

Yang Ting; Qiu Long; Huang Wanying; Xu Qianyi; Zou Jialing; Peng Qiding; Lin Honghui; Xi Dehui

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Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum

机译：辣椒静脉斑点病毒HCPRO与过氧化酯酶相互作用，以促进尼古利亚纳塔卡州病毒感染

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Plant symptoms are derived from specific interactions between virus and host components. However, little is known about viral or host factors that participate in the establishment of systemic necrosis. Here, we showed that helper component proteinase (HCPro), encoded by Chilli veinal mottle virus (ChiVMV), could directly interact with catalase 1 (CAT1) and catalase 3 (CAT3) in the cytoplasm of tobacco (Nicotiana tabacum) plants to facilitate viral infection. In vitro, the activities of CAT1 and CAT3 were inhibited by the interaction between HCPro and CATs. The C-terminus of HCPro was essential for their interaction and was also required for the decrease of enzyme activities. Interestingly, the mRNA and protein level of CATs were up-regulated in tobacco plants in response to ChiVMV infection. Nicotiana tabacum plants with HCPro overexpression or CAT1 knockout were more susceptible to ChiVMV infection, which was similar to the case of H2O2-pre-treated plants, and the overexpression of CAT1 inhibited ChiVMV accumulation. Also, neither CAT1 nor CAT3 could affect the RNA silencing suppression (RSS) activity of HCPro. Our results showed that the interaction between HCPro and CATs promoted the development of plant systemic necrosis, revealing a novel role for HCPro in virus infection and pathogenicity.

机译：植物症状来自病毒和宿主组件之间的特定相互作用。然而，对于参与建立全身性坏死的病毒或宿主因子知之甚少。在这里，我们展示了由辣椒静脉斑块病毒（ChiVMV）编码的辅助组分蛋白酶（HCPRO）可以直接与烟草（烟草塔巴帽）植物的细胞质中的过氧化氢酶1（CAT1）和过氧化氢酶3（CAT3）相互作用，以促进病毒感染。体外，CAT1和CAT3的活性被HCPRO和猫之间的相互作用抑制。 HCPRO的C-末端对于它们的相互作用至关重要，并且还需要减少酶活性。有趣的是，患有烟草的mRNA和蛋白质水平在烟草植物中响应ChiVMV感染而上调。 Nicotiana Tabacum植物与HCPRO过表达或CAT1敲除更容易受ChiVMV感染的影响，类似于H2O2-预处理的植物的情况，并且CAT1的过表达抑制了ChiVMV积累。此外，CAT1和CAT3都不会影响HCPRO的RNA沉默抑制（RSS）活性。我们的研究结果表明，HCPRO与猫之间的相互作用促进了植物系统性坏死的发展，揭示了在病毒感染和致病性中HCPRO的新作用。

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来源
《Journal of Experimental Botany》 |2020年第18期|共13页
作者
Yang Ting; Qiu Long; Huang Wanying; Xu Qianyi; Zou Jialing; Peng Qiding; Lin Honghui; Xi Dehui;
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作者单位

Sichuan Univ Coll Life Sci Key Lab Bioresource &

Ecoenvironm Minist Educ Chengdu 610065 Sichuan Peoples R China;

Sichuan Univ Coll Life Sci Key Lab Bioresource &

Ecoenvironm Minist Educ Chengdu 610065 Sichuan Peoples R China;

Sichuan Univ Coll Life Sci Key Lab Bioresource &

Ecoenvironm Minist Educ Chengdu 610065 Sichuan Peoples R China;

Sichuan Univ Coll Life Sci Key Lab Bioresource &

Ecoenvironm Minist Educ Chengdu 610065 Sichuan Peoples R China;

Sichuan Univ Coll Life Sci Key Lab Bioresource &

Ecoenvironm Minist Educ Chengdu 610065 Sichuan Peoples R China;

Sichuan Univ Coll Life Sci Key Lab Bioresource &

Ecoenvironm Minist Educ Chengdu 610065 Sichuan Peoples R China;

Sichuan Univ Coll Life Sci Key Lab Bioresource &

Ecoenvironm Minist Educ Chengdu 610065 Sichuan Peoples R China;

Sichuan Univ Coll Life Sci Key Lab Bioresource &

Ecoenvironm Minist Educ Chengdu 610065 Sichuan Peoples R China;

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原文格式 PDF
正文语种 eng
中图分类植物学;
关键词
Catalase; Chilli veinal mottle virus; HCPro; Nicotiana tabacum; suppressor of RNA silencing; systemic necrosis;

机译：过氧化氢酶;辣椒静脉斑点病毒;HCPRO;尼古利亚娜Tabacum;RNA沉默的抑制剂;系统坏死;

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专利

1. Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum [J] . Yang Ting, Qiu Long, Huang Wanying, Journal of Experimental Botany . 2020,第18期

机译：辣椒静脉斑点病毒HCPRO与过氧化酯酶相互作用，以促进尼古利亚纳塔卡州病毒感染
2. The role of phytochromes in Nicotiana tabacum against Chilli veinal mottle virus [J] . Fei Chunyan, Chen Lijuan, Yang Ting, Plant Physiology and Biochemistry . 2019,第期

机译：Phytochromes在尼古利尼宫斑驳烟草对辣椒甲状腺肿病毒的作用
3. Cucumber Mosaic Virus and Chili Veinal Mottle Virus Infection on Growth and Yield Component of Chilli [J] . DWI SUBEKTI, SRI HENDRASTUTI HIDAYAT, ENDANG NURHAYATI, Journal of biosciences . 2006,第2期

机译：黄瓜花叶病毒和辣椒斑驳病毒感染对辣椒生长和产量构成的影响
4. Heterologous encapsidation of potyviruses in mixed infections and in transgenic Nicotiana benthamiana expressing the coat protein gene of plum pox potyvirus (PPV) [C] . Edgar Maiss, D.-E. Lesemann, R. Koenig Key biosafety aspects of genetically modified organisms . 1995

机译：在混合感染和表达李子痘病毒（PPV）外壳蛋白基因的转基因烟草（Nicotiana benthamiana）中，马铃薯病毒的异源衣壳化
5. Cultured peanut (Arachis hypogaea L.) and tobacco (Nicotiana tabacum L.) cells infected with tomato spotted wilt virus for virus and vector studies [D] . Schuster, Greta Lucille. 1994

机译：培养的感染番茄斑点枯萎病毒的花生（Arachis hypogaea L.）和烟草（Nicotiana tabacum L.）细胞用于病毒和载体研究
6. Genetic diversity of Chilli veinal mottle virus infecting different chilli landraces in North East India indicates the possibility of transboundary movement of virus [O] . Rakesh Sanabam, Ng. Taibangnganbi Chanu, Susheel Kumar Sharma, 2018

机译：感染印度东北部不同辣椒地方品种的辣椒斑驳病毒的遗传多样性表明该病毒可能越境转移
7. First report of Pepper veinal mottle virus , Pepper yellows virus and a novel enamovirus in chilli pepper ( Capsicum sp.) in Rwanda [O] . A. Skelton, B. Uzayisenga, A. Fowkes, 2018

机译：辣椒呜咽斑点病毒，胡椒黄色病毒和辣椒（Capsicum sp）的新型搪瓷病毒的第一份报告

Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum

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