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首页> 外文期刊>Journal of Experimental Botany >Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato
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Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato

机译：细胞壁转化酶基因CIN1的异位过度表达导致番茄中的脱水避免

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摘要

Drought stress conditions modify source-sink relations, thereby influencing plant growth, adaptive responses, and consequently crop yield. Invertases are key metabolic enzymes regulating sink activity through the hydrolytic cleavage of sucrose into hexose monomers, thus playing a crucial role in plant growth and development. However, the physiological role of invertases during adaptation to abiotic stress conditions is not yet fully understood. Here it is shown that plant adaptation to drought stress can be markedly improved in tomato (Solanum lycopersicum L.) by overexpression of the cell wall invertase (cwInv) gene CIN1 from Chenopodium rubrum. CIN1 overexpression limited stomatal conductance under normal watering regimes, leading to reduced water consumption during the drought period, while photosynthetic activity was maintained. This caused a strong increase in water use efficiency (up to 50%), markedly improving water stress adaptation through an efficient physiological strategy of dehydration avoidance. Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants. However, the CIN1-overexpressing plants registered 3- to 6-fold higher cwInv activity in all analysed conditions. Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation. The induced sink metabolism in the leaves explained the maintenance of photosynthetic activity, delayed senescence, and increased source activity under drought stress. Moreover, CIN1 plants also presented a better control of production of reactive oxygen species and sustained membrane protection. Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves. Thus, cwInv critically functions at the integration point of metabolic, hormonal, and stress signals, providing a novel strategy to overcome drought-induced limitations to crop yield, without negatively affecting plant fitness under optimal growth conditions.
机译：干旱胁迫条件改变源汇关系，从而影响植物生长，适应性反应，并因此作物产量。倒置是通过蔗糖的水解裂解调节沉淀活性的关键代谢酶，进入己糖单体，从而在植物生长和发育中发挥着至关重要的作用。然而，倒转移在适应非生物胁迫条件期间的生理作用尚不完全理解。在这里，结果表明，通过从辛泛钙葡萄酒中的细胞壁转化酶（CWINV）基因CIN1的细胞壁转化酶（CWINV）基因CIN1的过表达，番茄（Solanum Lycopersicum L.）中可以显着改善植物适应。 CIN1过度表达在正常浇水方案下的气孔导度，导致干旱期间的耗水量降低，而育种活性则得到了光合活性。这引起了水利用效率的强劲增加（高达50％），通过脱水避免的有效生理策略显着提高水胁迫适应。干旱胁迫强烈降低CWINV活性，并在野生型植物的叶片中诱导其蛋白质抑制剂。然而，在所有分析的条件下，Cin1过度抑制植物在所有分析的条件下注册了3至6倍的CWINV活性。令人惊讶的是，由于初级代谢和水剥夺水平增加的糖 - 磷酸盐中间体的关键酶的活性，增强的转化酶活性不会导致代谢碳水化合物通量的激活增加增加的己糖浓度增加。叶片中诱导的水槽代谢解释了在干旱胁迫下维持光合活性，延迟衰老和增加的源活性。此外，CIN1植物还更好地控制了活性氧物质和持续膜保护的生产。 CIN1过表达赋予的那些代谢变化伴随着衰老延迟激素反式酶浓度的浓度增加，并在叶中衰老诱导乙烯前体1-氨基环丙烷-1-羧酸（ACC）降低。因此，CWINV在代谢，激素和应力信号的集成点处致力于克服新的策略来克服干旱诱导的作物产量的局限性，而不会在最佳生长条件下对植物适应性产生负面影响。

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