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首页> 外文期刊>Journal of Experimental Botany >Cadmium-inducible expression of the ABC-type transporter AtABCC3 increases phytochelatin-mediated cadmium tolerance in Arabidopsis
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Cadmium-inducible expression of the ABC-type transporter AtABCC3 increases phytochelatin-mediated cadmium tolerance in Arabidopsis

机译：ABC型转运蛋白ATABCC3的镉诱导的表达增加了拟南芥中的植物素介导的镉耐受性

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摘要

The heavy metal cadmium (Cd) is a widespread environmental contaminant with harmful effects on living cells. In plants, phytochelatin (PC)-dependent Cd detoxification requires that PC-Cd complexes are transported into vacuoles. Here, it is shown that Arabidopsis thaliana seedlings defective in the ABCC transporter AtABCC3 (abcc3) have an increased sensitivity to different Cd concentrations, and that seedlings overexpressing AtABCC3 (AtABCC3ox) have an increased Cd tolerance. The cellular distribution of Cd was analysed in protoplasts from abcc3 mutants and AtABCC3 overexpressors grown in the presence of Cd, by means of the Cd-specific fluorochromes 5-nitrobenzothiazole coumarin (BTC-5N) and Leadmium (TM) Green AM dye. This analysis revealed that Cd is mostly localized in the cytosol of abcc3 mutant protoplasts whereas there is an increase in vacuolar Cd in protoplasts from AtABCC3ox plants. Overexpression of AtABCC3 in cad1-3 mutant seedlings defective in PC production and in plants treated with l-buthionine sulphoximine (BSO), an inhibitor of PC biosynthesis, had no effect on Cd tolerance, suggesting that AtABCC3 acts via PCs. In addition, overexpression of AtABCC3 in atabcc1 atabcc2 mutant seedlings defective in the Cd transporters AtABCC1 and AtABCC2 complements the Cd sensitivity of double mutants, but not in the presence of BSO. Accordingly, the level of AtABCC3 transcript in wild type seedlings was lower than that of AtABCC1 and AtABCC2 in the absence of Cd but higher after Cd exposure, and even higher in atabcc1 atabcc2 mutants. The results point to AtABCC3 as a transporter of PC-Cd complexes, and suggest that its activity is regulated by Cd and is co-ordinated with the activity of AtABCC1/AtABCC2.
机译：重金属镉（CD）是一种普遍的环境污染物，对活细胞有害影响。在植物中，植物素（PC） - 依赖性CD解毒要求将PC-CD复合物转化为真空。这里，显示ABCC转运蛋白ATABCC3（ABCC3）缺陷的拟南芥幼苗对不同CD浓度的敏感性增加，并且过表达ATABCC3（ATABCC3OX）的幼苗具有增加的CD耐受性。通过在CD存在下从ABCC3突变体和ATABCC3过表达的原生质体中分析CD的细胞分布，借助于CD特异性荧光噻唑和硝基噻唑香豆素（BTC-5N）和碲（TM）绿色染料。该分析显示CD大部分位于ABCC3突变体原生质体的细胞溶胶中，而来自ATABCC3OX植物的原生质体中存在较大的真空CD。在CAD1-3突变体幼苗中的ATABCC3的过度表达在PC生产和用L-苯胺磺酰胺（BSO）处理的植物中，PC生物合成的抑制剂对Cd耐受性没有影响，表明ATABCC3通过PCS作用。此外，在CD转运蛋白ATABCC1和ATABCC2中的ATABCC1 ATABCC2突变幼苗中的ATABCC3的过表达是双突变体的CD敏感性，但在BSO的存在下，涉及双突变体的CD敏感性。因此，在野生型幼苗中的ATABCC3转录物的水平低于ATABCC1和ATABCC2，在不存在CD后，在CD暴露后较高，并且在ATABCC1 ATABCC2突变体中甚至更高。结果指向ATABCC3作为PC-CD复合物的转运蛋白，并表明其活性由CD调节，并与ATABCC1 / ATABCC2的活性协调。

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