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首页> 外文期刊>Journal of Experimental Botany >Stacks off tracks: a role for the golgin AtCASP in plant endoplasmic reticulum-Golgi apparatus tethering
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Stacks off tracks: a role for the golgin AtCASP in plant endoplasmic reticulum-Golgi apparatus tethering

机译：堆叠轨道：植物内质网 - 高尔基仪器卧室床上的床ATCASP的作用

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摘要

The plant Golgi apparatus modifies and sorts incoming proteins from the endoplasmic reticulum (ER) and synthesizes cell wall matrix material. Plant cells possess numerous motile Golgi bodies, which are connected to the ER by yet to be identified tethering factors. Previous studies indicated a role for cis-Golgi plant golgins, which are long coiled-coil domain proteins anchored to Golgi membranes, in Golgi biogenesis. Here we show a tethering role for the golgin AtCASP at the ER-Golgi interface. Using live-cell imaging, Golgi body dynamics were compared in Arabidopsis thaliana leaf epidermal cells expressing fluorescently tagged AtCASP, a truncated AtCASP-Delta CC lacking the coiled-coil domains, and the Golgi marker STtmd. Golgi body speed and displacement were significantly reduced in AtCASP-Delta CC lines. Using a dual-colour optical trapping system and a TIRF-tweezer system, individual Golgi bodies were captured in planta. Golgi bodies in AtCASP-Delta CC lines were easier to trap and the ER-Golgi connection was more easily disrupted. Occasionally, the ER tubule followed a trapped Golgi body with a gap, indicating the presence of other tethering factors. Our work confirms that the intimate ER-Golgi association can be disrupted or weakened by expression of truncated AtCASP-Delta CC and suggests that this connection is most likely maintained by a golgin-mediated tethering complex.
机译：植物GOLGI装置从内质网（ER）中改变并分类进入蛋白质并合成细胞壁基质材料。植物细胞具有许多动机Golgi体，其通过尚未被鉴定为彼得。以前的研究表明了CIS-GOLGI植物胶林的作用，该植物植物在Golgi生物发生器中锚定的长卷绕式卷域蛋白。在这里，我们对ER-GOLGI接口的Golgin ATCASP显示了一个束缚作用。使用Live-Cell成像，在拟南芥叶片表皮细胞中比较Golgi身体动态，表达荧光标记的ATCASP，缺乏卷绕式线圈结构域的截短的ATCASP-DELTA CC，以及GOLGI标记STTMD。 ATCASP-DELTA CC线大大降低了Golgi体速和位移。使用双色光学捕获系统和TiRF-Tweezer系统，在Planta中捕获单个高尔基体。 ATCASP-DELTA CC线路中的高尔基体更容易陷阱，并且更容易破坏ER-GOLGI连接。偶尔，ER小管遵循具有间隙的被困的高尔基体，表明存在其他束缚因子。我们的作品证实，通过表达截短的ATCASP-DERTA CC表达可以破坏或削弱INTIMAT ER-GOLGI协会，并表明这种联系最有可能由眼睑介导的培养物复合物维持。

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