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首页> 外文期刊>Journal of Experimental Botany >Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3
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Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3

机译：Citrus Citnac62与CitWrky1合作，通过Citaco3的上调参与柠檬酸降解

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Citric acid is the predominant organic acid of citrus fruit. Degradation of citric acid occurs during fruit development, influencing fruit acidity. Associations of CitAco3 transcripts and citric acid degradation have been reported for citrus fruit. Here, transient overexpression of CitAco3 significantly reduced the citric acid content of citrus leaves and fruits. Using dual luciferase assays, it was shown that CitNAC62 and CitWRKY1 could transactivate the promoter of CitAco3. Subcellular localization results showed that CitWRKY1 was located in the nucleus and CitNAC62 was not. Yeast two-hybrid analysis and bimolecular fluorescence complementation (BiFC) assays indicated that the two differently located transcription factors could interact with each other. Furthermore, BiFC showed that the protein-protein interaction occurred only in the nucleus, indicating the potential mobility of CitNAC62 in plant cells. A synergistic effect on citrate content was observed between CitNAC62 and CitWRKY1. Transient overexpression of CitNAC62 or CitWRKY1 led to significantly lower citrate content in citrus fruit. The combined expression of CitNAC62 and CitWRKY1 resulted in lower citrate content compared with the expression of CitNAC62 or CitWRKY1 alone. The transcript abundance of CitAco3 was consistent with the citrate content. Thus, we propose that a complex of CitWRKY1 and CitNAC62 contributes to citric acid degradation in citrus fruit, potentially via modulation of CitAco3.
机译：柠檬酸是柑橘类果实的主要有机酸。在水果发育过程中发生柠檬酸的降解，影响水果酸性。据报道，柠檬酸转录物和柠檬酸降解的缔约国果实果实。这里，冠军的瞬时过度表达显着降低了柑橘叶和水果的柠檬酸含量。使用双荧光素酶测定，结果表明，CitNAC62和CitWrky1可以转移冠军的启动子3。亚细胞定位结果表明，CITWRKY1位于核中，CITNAC62不是。酵母双杂化分析和双分子荧光互补（BIFC）测定表明，两个不同定位的转录因子可以彼此相互作用。此外，BIFC表明，仅在核中发生蛋白质 - 蛋白质相互作用，表明植物细胞中Citnac62的潜在迁移率。在Citnac62和CitWrky1之间观察到对柠檬酸盐含量的协同作用。 CITNAC62或CITWRKY1的瞬态过度表达导致柑橘类水果中的柠檬酸含量显着降低。与单独的CitNac62或CitWrky1的表达相比，Citnac62和CitWrky1的组合表达导致柠檬酸含量较低。 CITACO3的转录物丰度与柠檬酸含量一致。因此，我们提出的是Citwrky1和Citnac62的复合物有助于柑橘类果实中的柠檬酸降解，可能通过调节Citaco3。

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