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首页> 外文期刊>Journal of Experimental Botany >EMPTY PERICARP11 serves as a factor for splicing of mitochondrial nad1 intron and is required to ensure proper seed development in maize
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EMPTY PERICARP11 serves as a factor for splicing of mitochondrial nad1 intron and is required to ensure proper seed development in maize

机译：空的Pericarp11作为剪接线粒体NAD1内含子的因素，并且需要确保玉米种子

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摘要

Group II introns are common in the mitochondrial genome of higher plant species. The splicing of these introns is a complex process involving the synergistic action of multiple factors. However, few of these factors have been characterized in maize. In this study, we found that the Empty pericarp11 (Emp11) gene, which encodes a P-type pentatricopeptide repeat (PPR) protein, is required for the development of maize seeds. The loss of Emp11 function seriously impairs embryo and endosperm development, resulting in empty pericarp seeds in maize, and alteration in Emp11 expression leads to quantitative variation in kernel size and weight. We found that the emp11 mutants showed a failure in nad1 intron splicing, exhibited a severe reduction in complex I assembly and activity, mitochondrial structure disturbances, and an increase in alternative oxidase AOX2 and AOX3 levels. Interestingly, the emp11 phenotype was very severe in the W22 inbred line but could be partially recovered in B73 BC2F2 segregating ears. These results suggest that EMP11 serves as a factor for the splicing of mitochondrial nad1 introns and is required for mitochondrial function to ensure proper seed development in maize.
机译：II组内含子在高等植物种类的线粒体基因组中常见。这些内含子的拼接是涉及多种因素的协同作用的复杂过程。然而，很少有这些因素已经在玉米中表征。在这项研究中，我们发现，对于玉米种子的发育，需要编码p型五丙二肽重复（PPR）蛋白的空皮PERICARP11（EMP11）基因。 EMP11功能的丧失严重损害胚胎和胚乳的发育，导致玉米中的空皮种子，并且EMP11表达的改变导致核尺寸和重量的定量变化。我们发现EMP11突变体显示NAD1内含子剪接的失败，表现出复杂的I组装和活性，线粒体结构干扰的严重降低，以及替代氧化酶AOX2和AOX3水平的增加。有趣的是，EMP11表型在W22近交系中非常严重，但可以在B73 BC2F2隔离耳中部分回收。这些结果表明EMP11用作线粒体NAD1内含子的剪接的因素，并且是线粒体功能所必需的，以确保玉米中适当的种子发育。

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