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首页> 外文期刊>Journal of Experimental Botany >Green-fruited Solanum habrochaites lacks fruit-specific carotenogenesis due to metabolic and structural blocks
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Green-fruited Solanum habrochaites lacks fruit-specific carotenogenesis due to metabolic and structural blocks

机译：由于代谢和结构块，绿色荞可含辣椒凋亡缺乏果实特异性病变

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摘要

Members of the tomato clade exhibit a wide diversity in fruit color, but the mechanisms governing inter-species diversity of coloration are largely unknown. The carotenoid profiles, carotenogenic gene expression and proteome profiles of green-fruited Solanum habrochaites (SH), orange-fruited S. galapagense, and red-fruited S. pimpinellifolium were compared with cultivated tomato [S. lycopersicum cv. Ailsa Craig (SL)] to decipher the molecular basis of coloration diversity. Green-fruited SH, though it showed normal expression of chromoplast-specific phytoene synthase1 and lycopene beta-cyclase genes akin to orange/red-fruited species, failed to accumulate lycopene and beta-carotene. The SH phytoene synthase1 cDNA encoded an enzymatically active protein, whereas the lycopene beta-cyclase cDNA was barely active. Consistent with its green-fruited nature, SH's fruits retained chloroplast structure and PSII activity, and had impaired chlorophyll degradation with high pheophorbide a levels. Comparison of the fruit proteomes with SL revealed retention of the proteome complement related to photosynthesis in SH. Targeted peptide monitoring revealed a low abundance of key carotenogenic and sequestration proteins in SH compared with tomato. The green-fruitedness of SH appears to stem from blocks at several critical steps regulating fruit-specific carotenogenesis namely the absence of chloroplast to chromoplast transformation, block in carotenoid biosynthesis, and a dearth of carotenoid sequestering proteins.
机译：番茄思想的成员在果实颜色上表现出广泛的多样性，但管理物种间着色的机制很大程度上是未知的。与培养的番茄相比，与绿色溶素母羊毛草（SH），橙色Fruited S.Alapagense和Red-Fruited的S.Pimpinellifolium相比，雌突出的基因表达和蛋白质组谱系的类胡萝卜素谱系，雌激素基因表达和蛋白质组谱进行了比较[S. Lycopersicum cv。 AILSA CRAIG（SL）]破译着色分集的分子基础。绿色气体静脉，虽然它表现出染色体特异性植物合酶的正常表达和类似于橙色/红色的物种的番茄鸡β-环酶基因，但未能积累番茄红素和β-胡萝卜素。 SH植物合酶1 cDNA编码了一种酶活性蛋白质，而番茄红素β-环酶cDNA几乎没有活性。 SH的水果保留了叶绿体结构和PSII活性，致力于致氯化物的叶绿体结构和PSII活性。 SL的水果蛋白质的比较显示了与SH中的光合作用相关的蛋白质组补充的保留。靶向肽监测揭示了与番茄相比，SH中的重点雌激素和螯合蛋白的低丰度。 SH的绿色助手似乎在若干关键步骤中源于调节果实特异性雌激素的块状，即没有叶绿体转化的叶绿体，在类胡萝卜素生物合成中，以及类胡萝卜素螯合蛋白的缺乏。

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