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首页> 外文期刊>Journal of Experimental Botany >Integrative analysis of the late maturation programme and desiccation tolerance mechanisms in intermediate coffee seeds
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Integrative analysis of the late maturation programme and desiccation tolerance mechanisms in intermediate coffee seeds

机译：中间咖啡种子晚期成熟程序和干燥耐受机制的整合分析

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摘要

The 'intermediate seed' category was defined in the early 1990s using coffee (Coffea arabica) as a model. In contrast to orthodox seeds, intermediate seeds cannot survive complete drying, which is a major constraint for seed storage and has implications for both biodiversity conservation and agricultural purposes. However, intermediate seeds are considerably more tolerant to drying than recalcitrant seeds, which are highly sensitive to desiccation. To gain insight into the mechanisms governing such differences, changes in desiccation tolerance (DT), hormone contents, and the transcriptome were analysed in developing coffee seeds. Acquisition of DT coincided with a dramatic transcriptional switch characterised by the repression of primary metabolism, photosynthesis, and respiration, and the up-regulation of genes coding for late-embryogenesis abundant (LEA) proteins, heat-shock proteins (HSPs), and antioxidant enzymes. Analysis of the heat-stable proteome in mature coffee seeds confirmed the accumulation of LEA proteins identified at the transcript level. Transcriptome analysis also suggested a major role for ABA and for the transcription factors CaHSFA9, CaDREB2G, CaANAC029, CaPLATZ, and CaDOG-like in DT acquisition. The ability of CaHSFA9 and CaDREB2G to trigger HSP gene transcription was validated by Agrobacterium-mediated transformation of coffee somatic embryos.
机译：“中间种子”类别在20世纪90年代初期定义使用咖啡（Coffea Arabica）作为模型。与正统种子相比，中间种子不能存活完全干燥，这是种子储存的主要限制，并对生物多样性保护和农业目的有影响。然而，中间体种子比醋酸种子更容许干燥，这对醋酸种子高度敏感。为了深入了解治疗这种差异的机制，在显影咖啡种子中分析了干燥耐受性（DT），激素含量和转录组的变化。采集DT与剧烈转录开关相一致，其特征在于抑制初级代谢，光合作用和呼吸，以及编码晚期胚胎发生的基因的上调，植物蛋白质，热冲击蛋白（HSP）和抗氧化剂酶。成熟咖啡种子中热稳定蛋白质组的分析证实了在转录物水平上鉴定的Lea蛋白的积累。转录组分析还提出了ABA和转录因子Cahsfa9，Cadreb2g，Caanac029，Caplatz和Cadog样在DT采集中的主要作用。 Cahsfa9和Cadreb2g触发HSP基因转录的能力通过农杆菌介导的咖啡胚胎胚胎的转化验证。

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