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首页> 外文期刊>Journal of Experimental Botany >Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis
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Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis

机译：拟南芥中高通量突变体筛选的超快速生长型促进剂分析

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摘要

Auxin (indole-3-acetic acid, IAA) plays fundamental roles as a signalling molecule during numerous plant growth and development processes. The formation of local auxin gradients and auxin maxima/minima, which is very important for these processes, is regulated by auxin metabolism (biosynthesis, degradation, and conjugation) as well as transport. When studying auxin metabolism pathways it is crucial to combine data obtained from genetic investigations with the identification and quantification of individual metabolites. Thus, to facilitate efforts to elucidate auxin metabolism and its roles in plants, we have developed a high-throughput method for simultaneously quantifying IAA and its key metabolites in minute samples (&10 mg FW) of Arabidopsis thaliana tissues by in-tip micro solid-phase extraction and fast LC-tandem MS. As a proof of concept, we applied the method to a collection of Arabidopsis mutant lines and identified lines with altered IAA metabolite profiles using multivariate data analysis. Finally, we explored the correlation between IAA metabolite profiles and IAA-related phenotypes. The developed rapid analysis of large numbers of samples (&100 samples d(-1)) is a valuable tool to screen for novel regulators of auxin metabolism and homeostasis among large collections of genotypes.
机译：蟾蜍素（吲哚-3-醋酸，IAA）在众多植物生长和发育过程中起着基本作用作为信号分子。局部植物素梯度和养蛋白最大值/最小值的形成是由毒素代谢（生物合成，降解和缀合）和运输的调节。在研究生长素的代谢途径时，将从遗传调查中获得的数据与个体代谢物的鉴定和定量结合起来至关重要。因此，为了促进努力阐明植物中的植物中的疾病代谢及其作用，我们开发了一种高通量的方法，用于同时定量IAA及其在分钟样品（＆amp; LT; 10毫克FW）的拟南芥组织中的关键代谢物。尖端微固相提取和快速LC串联MS。作为概念证明，我们将该方法应用于拟南芥突变线的集合，并使用多元数据分析鉴定了具有改变的IAA代谢物谱的线。最后，我们探讨了IAA代谢物谱与IAA相关表型之间的相关性。大量样品的快速分析（＆amp; 100样品D（-1））是一种有价值的工具，用于筛选疾病新陈代谢和稳态的新型基因型中的促进剂新陈代谢。

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  • 来源
    《Journal of Experimental Botany》 |2018年第10期|共11页
  • 作者

    Pencik Ales; Casanova-Saez Ruben; Pilarova Veronika; Zukauskaite Asta; Pinto Rui; Micol Jose Luis; Ljung Karin; Novak Ondrej;

  • 作者单位

    Swedish Univ Agr Sci Dept Forest Genet &

    Plant Physiol Umea Plant Sci Ctr SE-90183 Umea Sweden;

    Swedish Univ Agr Sci Dept Forest Genet &

    Plant Physiol Umea Plant Sci Ctr SE-90183 Umea Sweden;

    Charles Univ Prague Fac Pharm Hradec Kralove Dept Analyt Chem Heyrovskeho 1203 CZ-50005 Hradec Kralove Czech Republic;

    Palacky Univ Fac Sci Ctr Reg Hana Biotechnol &

    Agr Res Lab Growth Regulators Slechtitelu 27 CZ-78371 Olomouc Czech Republic;

    Umea Univ Chem Dept KBC Computat Life Sci Cluster CLiC SE-90187 Umea Sweden;

    Univ Miguel Hernandez Inst Bioingn Campus Elche Alicante 03202 Spain;

    Swedish Univ Agr Sci Dept Forest Genet &

    Plant Physiol Umea Plant Sci Ctr SE-90183 Umea Sweden;

    Swedish Univ Agr Sci Dept Forest Genet &

    Plant Physiol Umea Plant Sci Ctr SE-90183 Umea Sweden;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    Arabidopsis thaliana; auxin; metabolite profiling; multivariate data analysis; mutant; screening;

    机译：Arabidopsis thaliana;植物;代谢物分析;多变量数据分析;突变体;筛选;

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