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首页> 外文期刊>Journal of Experimental Botany >The Arabidopsis COPII components, AtSEC23A and AtSEC23D, are essential for pollen wall development and exine patterning
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The Arabidopsis COPII components, AtSEC23A and AtSEC23D, are essential for pollen wall development and exine patterning

机译：Arabidopsis Copii组件，ATSEC23A和ATSEC23D对于花粉墙开发和外出图案来说是必不可少的

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The specialized multilayered pollen wall plays multiple roles to ensure normal microspore development. The major components of the pollen wall (e.g. sporopollenin and lipidic precursors) are provided from the tapetum. Material export from the endoplasmic reticulum (ER) is mediated by coat protein complex II (COPII) vesicles. The Arabidopsis thaliana genome encodes seven homologs of SEC23, a COPII component. However, the functional importance of this diversity remains elusive. Here, we analyzed knockout and knockdown lines for AtSEC23A and AtSEC23D, two of the A. thaliana SEC23 homologs, respectively. Single atsec23a and atsec23d mutant plants, despite normal fertility, showed an impaired exine pattern. Double atsec23ad mutant plants were semi-sterile and exhibited developmental defects in pollen and tapetal cells. Pollen grains of atsec23ad had defective exine and intine, and showed signs of cell degeneration. Moreover, the development of tapetal cells was altered, with structural abnormalities in organelles. AtSEC23A and AtSEC23D exhibited the characteristic localization pattern of COPII proteins and were highly expressed in the tapetum. Our work suggests that AtSEC23A and AtSEC23D may organize pollen wall development and exine patterning by regulating ER export of lipids and proteins necessary for pollen wall formation. Also, our results shed light on the functional heterogeneity of SEC23 homologs.
机译：专业的多层花粉墙发挥了多种作用，以确保正常的微孔发育。花粉壁的主要成分（例如孢子庚烯素和脂质前体）提供从翅膀提供。从内质网（ER）的物质导出由外套蛋白质复合物II（COPII）囊泡介导。拟南芥基因组编码Sec23的七种同源物，COPII组分。然而，这种多样性的功能重要性仍然难以捉摸。在这里，我们分析了ATSEC23A和ATSEC23D的淘汰和敲除，分别是A. Thilala Sec23同源物中的两种。唯一ATSEC23A和ATSEC23D突变植物，尽管生育率正常，表现出损害的外出模式。 Double Atsec23AD突变植物是半无菌的，并且在花粉和翅膀细胞中表现出发育缺陷。 ATSEC23AD的花粉颗粒有缺陷的外部和内部，并显示细胞变性的迹象。此外，胶质细胞的发展被改变，具有细胞器的结构异常。 ATSEC23A和ATSEC23D表现出COPII蛋白的特征定位模式，并且在Tapetum中高度表达。我们的工作表明，ATSEC23A和ATSEC23D可以通过调节花粉壁形成所需的脂质和蛋白质的ER出口来组织花粉墙开发和外来图案。此外，我们的结果阐明了Sec23同源物的功能异质性。

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