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首页> 外文期刊>Journal of Experimental Botany >Reduced Anthocyanins in Petioles codes for a GST anthocyanin transporter that is essential for the foliage and fruit coloration in strawberry
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Reduced Anthocyanins in Petioles codes for a GST anthocyanin transporter that is essential for the foliage and fruit coloration in strawberry

机译：降低叶柄中的花青素，用于GST花青素转运蛋白，这对于草莓中的叶子和果实着色是必不可少的

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摘要

The red color of the foliage and fruit in strawberry comes from anthocyanins stored in the vacuole; however, how this anthocyanin accumulation is regulated remains unclear. A reduced anthocyanin in petioles (rap) mutant was identified in an N-ethyl-N-nitrosourea (ENU) mutagenized population of YW5AF7, a white-fruited variety of the wild strawberry Fragaria vesca. The causative mutation was identified to be a premature stop codon in a glutathione S-transferase (GST) gene. In addition to the foliage coloration, RAP also mediates fruit pigmentation and acts downstream of the fruit-specific transcription factor FvMYB10. Among all eight GST genes in the same subfamily, RAP is most abundantly expressed in the ripening fruit. Expression analysis and transient expression assays demonstrated that RAP is the principal transporter of anthocyanins among the paralogs. Moreover, domain-swap experiments showed that both the N-and C-terminals of RAP are essential for the binding capability of anthocyanins. In addition, transient knock-down of RAP resulted in reduced fruit coloration in cultivated strawberry. Collectively, our results demonstrate that RAP encodes the principal GST transporter of anthocyanins in the strawberry foliage and fruit, and it could be modified to alter the fruit color in strawberry.
机译：草莓中叶子和果实的红颜色来自储液中的花青素;然而，这种花青素积累是如何监管的仍然不清楚。在N-乙基-N-亚硝基脲（ENU）诱变的YW5AF7诱变群体中，鉴定了叶片（RAP）突变体中的降低的花青素，是野生草莓Fragaria vesca的白色碎片。鉴定原因突变是谷胱甘肽S-转移酶（GST）基因中的过早止血密码子。除了叶子着色外，RAP还在果实色素沉着中介导果实色素沉着，并作用在果实特异性转录因子FVMYB10的下游。在同一亚家族中的所有八个GST基因中，RAP在成熟的果实中最大限度地表达。表达分析和瞬时表达测定表明，RAP是帕拉氯葡萄球菌中花青素的主要转运蛋白。此外，结构域 - 交换实验表明，RAP的N和C末端对于花青素的结合能力至关重要。此外，RAP的瞬态敲击导致栽培草莓中的果色着色降低。统称，我们的结果表明，RAP编码了草莓叶子和水果中花青素的主要GST转运蛋白，可以修改以改变草莓中的果实颜色。

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