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首页> 外文期刊>Journal of Experimental Botany >The Nicotiana tabacum ABC transporter NtPDR3 secretes O-methylated coumarins in response to iron deficiency
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The Nicotiana tabacum ABC transporter NtPDR3 secretes O-methylated coumarins in response to iron deficiency

机译：Nicotiana Tabacum ABC Transporter NTPDR3响应铁缺乏症分泌O-甲基化香豆素

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摘要

Although iron is present in large amounts in the soil, its poor solubility means that plants have to use various strategies to facilitate its uptake. In this study, we show that expression of NtPDR3/NtABCG3, a Nicotiana tabacum plasma-membrane ABC transporter in the pleiotropic drug resistance (PDR) subfamily, is strongly induced in the root epidermis under iron deficiency conditions. Prevention of NtPDR3 expression resulted in N. tabacum plants that were less tolerant to iron-deficient conditions, displaying stronger chlorosis and slower growth than those of the wildtype when not supplied with iron. Metabolic profiling of roots and root exudates revealed that, upon iron deficiency, secretion of catechol-bearing O-methylated coumarins such as fraxetin, hydroxyfraxetin, and methoxyfraxetin to the rhizosphere was compromised in NtPDR3-silenced plants. However, exudation of flavins such as riboflavin was not markedly affected by NtPDR3-silencing. Expression of NtPDR3 in N. tabacum Bright Yellow-2 (BY-2) cells resulted in altered intra-and extracellular coumarin pools, supporting coumarin transport by this transporter. The results demonstrate that N. tabacum secretes both coumarins and flavins in response to iron deficiency and that NtPDR3 plays an essential role in the plant response to iron deficiency by mediating secretion of O-methylated coumarins to the rhizosphere.
机译：虽然铁在土壤中大量存在，但其贫困性差意味着植物必须使用各种策略来促进其摄取。在本研究中，我们表明NTPDR3 / NTABCG3的表达，烟草毒性抗性（PDR）血管膜ABC转运蛋白在铁缺乏条件下强烈诱导根表皮中的强烈诱导。预防NTPDR3表达导致N. Tabacum植物，耐铁缺乏条件较低，均比不提供铁的氯结源和较强的氯化和较慢的生长。根系和根部的代谢分析显示，在缺铁缺乏时，在NTPDR3-沉默的植物中损害了无脂素，羟基分布和甲氧基丙酮等儿茶素，羟基丙烯蛋白和甲氧基丙酮。然而，通过NTPDR3-沉默，如核黄素如核黄素如核黄素的渗出。 NTPDR3在N.Babacum亮黄-2（BY-2）细胞的表达导致改变的内含内和细胞外香豆素池，支持该转运蛋白的香豆素运输。结果表明，N. Tabacum患有对铁缺乏症的香豆素和黄曲品，并且NTPDR3通过将O-甲基化香豆素分泌到根际，在植物对缺铁的作用反应中起重要作用。

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  • 来源
    《Journal of Experimental Botany》 |2018年第18期|共13页
  • 作者

    Lefevre Francois; Fourmeau Justine; Pottier Mathieu; Baijot Amandine; Cornet Thomas; Abadia Javier; Alvarez-Fernandez Ana; Boutry Marc;

  • 作者单位

    Catholic Univ Louvain Louvain Inst Biomol Sci &

    Technol Croix Sud 4-5 Box L7-04-14 B-1348 Louvain La Neuve Belgium;

    Catholic Univ Louvain Louvain Inst Biomol Sci &

    Technol Croix Sud 4-5 Box L7-04-14 B-1348 Louvain La Neuve Belgium;

    Catholic Univ Louvain Louvain Inst Biomol Sci &

    Technol Croix Sud 4-5 Box L7-04-14 B-1348 Louvain La Neuve Belgium;

    Catholic Univ Louvain Louvain Inst Biomol Sci &

    Technol Croix Sud 4-5 Box L7-04-14 B-1348 Louvain La Neuve Belgium;

    Catholic Univ Louvain Louvain Inst Biomol Sci &

    Technol Croix Sud 4-5 Box L7-04-14 B-1348 Louvain La Neuve Belgium;

    CSIC Estac Expt Aula Dei Dept Plant Nutr Apdo 13034 E-50080 Zaragoza Spain;

    CSIC Estac Expt Aula Dei Dept Plant Nutr Apdo 13034 E-50080 Zaragoza Spain;

    Catholic Univ Louvain Louvain Inst Biomol Sci &

    Technol Croix Sud 4-5 Box L7-04-14 B-1348 Louvain La Neuve Belgium;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    ABC transporter; coumarins; flavins; iron deficiency; Nicotiana; root exudates;

    机译：ABC运输车;香豆素;黄酮;铁缺乏;尼古利亚纳;根渗出物;

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