...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >The SIFSR gene controls fruit shelf-life in tomato
【24h】

The SIFSR gene controls fruit shelf-life in tomato

机译：SIFSR基因控制番茄的水果保质期

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fruit ripening represents a process that changes flavor and appearance and also a process that dramatically increases fruit softening. Fruit softening and textural variations mainly result from disruptions to the cell walls of the fruit throughout ripening, but the exact mechanisms and specific modifications of the cell wall remain unclear. Plant-specific GRAS proteins play a critical role in development and growth. To date, few GRAS genes have been functionally categorized in tomato. The expression of a novel GRAS gene described in this study and designated as SIFSR (fruit shelf-life regulator) specifically increased during fruit ripening, but was significantly decreased in the tomato mutant rin (ripening inhibitor). RNAi repression of SIFSR resulted in reduced expression of multiple cell wall modification-related genes, decreased the activities of PG (polygalacturonase), TBG (tomato (beta-galactosidase), CEL (cellulase), and XYL (beta-D-xylosidase), and significantly prolonged fruit shelf-life. Furthermore, overexpression of SIFSR in mutant rin gave rise to up-regulated expression of multiple cell wall modification-related genes, such as PG, TBG4, CEL2, XYL1, PL, PE, MAN1, EXP1, and XTH5, and significantly shortened the fruit shelf-life. These findings reveal some of the genetic mechanisms underlying fruit cell wall metabolism and suggest that the SIFSR gene is another potential biotechnological target for the control of tomato fruit shelf-life.
机译：果实成熟代表了改变风味和外观的过程以及显着增加水果软化的过程。水果软化和纹理变化主要是由于整个成熟的果实中断的破坏，但细胞壁的确切机制和特定修饰仍然不清楚。植物特异性GRAS蛋白在开发和增长中发挥着关键作用。迄今为止，很少有GRAS基因在番茄中归类。在本研究中描述的新型GRAS基因并指定为SIFSR（果子素材寿命调节剂）在果实成熟过程中特别增加，但在番茄突变体载体（成熟抑制剂）中显着降低。 SIFSR的RNAi抑制导致多细胞壁改性相关基因的表达减少，降低了PG（多糖呋喃糖酶），TBG（番茄（甲基半乳糖苷酶），CEL（纤维素酶）和XYL（β-D-木糖苷酶）的活性的活性，并且显着延长果实保质期。此外，突变体rin中SIFSR的过度表达产生了多个细胞壁改性相关基因的上调表达，例如PG，TBG4，CEL2，XYL1，PL，PE，MAN1，EXP1，MAN1，EXP1，和xth5，并显着缩短了水果保质期。这些发现揭示了果细胞壁代谢的一些遗传机制，并表明SIFSR基因是控制番茄果实保质期的另一个潜在的生物技术靶标。

著录项

  • 来源
    《Journal of Experimental Botany》 |2018年第12期|共13页
  • 作者

    Zhang Lincheng; Zhu Mingku; Ren Lijun; Li Anzhou; Chen Guoping; Hu Zongli;

  • 作者单位

    Chongqing Univ Bioengn Coll Minist Educ Key Lab Biorheol Sci &

    Technol Chongqing 400044 Peoples R China;

    Chongqing Univ Bioengn Coll Minist Educ Key Lab Biorheol Sci &

    Technol Chongqing 400044 Peoples R China;

    Chongqing Univ Bioengn Coll Minist Educ Key Lab Biorheol Sci &

    Technol Chongqing 400044 Peoples R China;

    Chongqing Univ Bioengn Coll Minist Educ Key Lab Biorheol Sci &

    Technol Chongqing 400044 Peoples R China;

    Chongqing Univ Bioengn Coll Minist Educ Key Lab Biorheol Sci &

    Technol Chongqing 400044 Peoples R China;

    Chongqing Univ Bioengn Coll Minist Educ Key Lab Biorheol Sci &

    Technol Chongqing 400044 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    Cell wall metabolism; GRAS transcription factor; overexpression; RNAi; shelf-life; SIFSR; tomato;

    机译：细胞壁代谢;GRAS转录因子;过表达;RNAi;保质期;SIFSR;番茄;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号