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首页> 外文期刊>Journal of Experimental Botany >SiSTL1, encoding a large subunit of ribonucleotide reductase, is crucial for plant growth, chloroplast biogenesis, and cell cycle progression in Setaria italica
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SiSTL1, encoding a large subunit of ribonucleotide reductase, is crucial for plant growth, chloroplast biogenesis, and cell cycle progression in Setaria italica

机译：SIST11，编码核糖核苷酸还原酶的大亚基，对于植物生长，叶绿体生物发生和濑户内的细胞周期进展至关重要

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摘要

The activity of ribonucleotide reductase (RNR), which catalyses the transformation of four ribonucleoside diphosphates (NDPs) to their corresponding deoxyribonucleoside diphosphates (dNDPs), is the main determiner of the cellular concentration of dNTP pools and should be tightly coordinated with DNA synthesis and cell-cycle progression. Constitutively increased or decreased RNR activity interferes with DNA replication and leads to arrested cell cycle progression; however, the mechanisms underlying these disruptive effects in higher plants remain to be uncovered. In this study, we identified a RNR large subunit mutant, sistl1, in Setaria italica (foxtail millet), which exhibited growth retardation as well as striped leaf phenotype, i.e. irregularly reduced leaf vein distances and decreased chloroplast biogenesis. We determined that a Gly737 to Glu substitution occurring in the C-terminus of the SiSTL1 protein slightly affected its optimal function, leading in turn to the reduced expression of genes variously involved in the assembly and activation of the DNA pre-replicative complex, elongation of replication forks and S phase entry. Our study provides new insights into how SiSTL1 regulates plant growth, chloroplast biogenesis, and cell cycle progression in Poaceae crops.
机译：核糖核苷酸还原酶（RNR）的活性，其将四种核糖核苷二磷酸二磷酸（NDPS）转化为它们对应的脱氧核糖核苷二磷酸（DNDPS）的转化是DNTP池细胞浓度的主要测定率，并且应与DNA合成和细胞密切相关 - 循环进展。组成型增加或降低的RNR活性会使DNA复制干扰并导致被捕的细胞周期进展;然而，在高等植物中依据这些破坏性效应的机制仍未被发现。在该研究中，我们鉴定了血小套italica（Foxtail Millet）中的RNR大亚基突变体SIST11，其表现出生长延迟以及条纹叶片表型，即，不规则地降低叶静脉距离和降低叶绿体生物发生。我们确定在SISTL1蛋白的C-末端发生的Gly737略微影响其最佳功能，依次导致各种涉及组装和激活DNA预复制复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复合复制的复制叉子和S相条目。我们的研究提供了对SISTL1如何调节植物生长，叶绿体生物发生和细胞周期进展的新洞察力。

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