Sorghum qTGW1a encodes a G-protein subunit and acts as a negative regulator of grain size

Zou Guihua; Zhai Guowei; Yan Song; Li Sujuan; Zhou Lengbo; Ding Yanqing; Liu Heqin; Zhang Zhipeng; Zou Jianqiu; Zhang Liyi; Chen Junping; Xin Zhanguo; Tao Yuezhi

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Sorghum qTGW1a encodes a G-protein subunit and acts as a negative regulator of grain size

机译：高粱QTGW1A编码G蛋白亚基，并充当晶粒尺寸的负调节器

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Grain size is a major determinant of grain yield in sorghum and other cereals. Over 100 quantitative trait loci (QTLs) of grain size have been identified in sorghum. However, no gene underlying any grain size QTL has been cloned. Here, we describe the fine mapping and cloning of one grain size QTL. From an F-8 recombinant inbred line population derived from a cross between inbred lines 654 and LTR108, we identified 44 grain size QTLs. One QTL, qTGW1a, was detected consistently on the long arm of chromosome 1 in the span of 4 years. Using the extreme recombinants from an F-2:3 fine-mapping population, qTGW1a was delimited within a similar to 33 kb region containing three predicted genes. One of them, SORBI_3001G341700, predicted to encode a G-protein gamma subunit and homologous to GS3 in rice, is likely to be the causative gene for qTGW1a. qTGW1a appears to act as a negative regulator of grain size in sorghum. The functional allele of the putatively causative gene of qTGW1a from inbred line 654 decreased grain size, plant height, and grain yield in transgenic rice. Identification of the gene underlying qTGW1a advances our understanding of the regulatory mechanisms of grain size in sorghum and provides a target to manipulate grain size through genome editing.

机译：粒度是高粱和其他谷物籽粒产量的主要决定因素。在高粱中鉴定出超过100种定量特质基因座（QTL）。然而，没有克隆任何晶粒尺寸QTL的基因。在这里，我们描述了一种晶粒尺寸QTL的精细映射和克隆。来自衍生自交叉线654和LTR108之间的交叉的F-8重组近交线，我们识别44粒尺寸QTL。在4年的跨度，在染色体1的长臂上一致地检测一个QTL QTGW1A。使用来自F-2的极端重组剂：3细映射群，QTGW1a在类似于33kb的含有三个预测基因的情况下界定。其中一个，预测在水稻中编码G蛋白γ亚基和对GS3同源的索兰_3001G341700可能是QTGW1A的致病基因。 QTGW1A似乎充当高粱晶粒尺寸的负调节器。近红线654 QTGW1A的QTGW1A的稳定致病基因的功能等位基因减少了转基因水稻中的晶粒尺寸，植物高度和籽粒产量。 QTGW1A潜在的基因的鉴定进展了我们对高粱晶粒尺寸的调节机制的理解，并提供了通过基因组编辑操纵晶粒尺寸的靶标。

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来源
《Journal of Experimental Botany》 |2020年第18期|共13页
作者
Zou Guihua; Zhai Guowei; Yan Song; Li Sujuan; Zhou Lengbo; Ding Yanqing; Liu Heqin; Zhang Zhipeng; Zou Jianqiu; Zhang Liyi; Chen Junping; Xin Zhanguo; Tao Yuezhi;
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作者单位

Zhejiang Acad Agr Sci State Key Lab Managing Biot &

Chem Threats Qual &

Inst Crop &

Nucl Technol Utilizat Hangzhou 310021 Peoples R China;

Zhejiang Acad Agr Sci State Key Lab Managing Biot &

Chem Threats Qual &

Inst Crop &

Nucl Technol Utilizat Hangzhou 310021 Peoples R China;

Jiangxi Acad Agr Sci Rice Res Inst Rice Natl Engn Lab Nanchang 330200 Jiangxi Peoples R China;

Zhejiang Acad Agr Sci State Key Lab Managing Biot &

Chem Threats Qual &

Inst Crop &

Nucl Technol Utilizat Hangzhou 310021 Peoples R China;

Guizhou Acad Agr Sci Inst Upland Food Crops Guiyang 550009 Peoples R China;

Guizhou Acad Agr Sci Inst Upland Food Crops Guiyang 550009 Peoples R China;

Zhejiang Acad Agr Sci State Key Lab Managing Biot &

Chem Threats Qual &

Inst Crop &

Nucl Technol Utilizat Hangzhou 310021 Peoples R China;

Liaoning Acad Agr Sci Chinese Natl Sorghum Improvement Ctr Shenyang 110161 Peoples R China;

Liaoning Acad Agr Sci Chinese Natl Sorghum Improvement Ctr Shenyang 110161 Peoples R China;

Guizhou Acad Agr Sci Inst Upland Food Crops Guiyang 550009 Peoples R China;

USDA ARS Plant Stress &

Germplasm Dev Unit Cropping Syst Res Lab Lubbock TX 79415 USA;

USDA ARS Plant Stress &

Germplasm Dev Unit Cropping Syst Res Lab Lubbock TX 79415 USA;

Zhejiang Acad Agr Sci State Key Lab Managing Biot &

Chem Threats Qual &

Inst Crop &

Nucl Technol Utilizat Hangzhou 310021 Peoples R China;

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原文格式 PDF
正文语种 eng
中图分类植物学;
关键词
Cloning; fine mapping; grain size; overexpression; qTGW1a; sorghum;

机译：克隆;精细映射;粒度;过表达;QTGW1A;高粱;

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专利

1. Sorghum qTGW1a encodes a G-protein subunit and acts as a negative regulator of grain size [J] . Zou Guihua, Zhai Guowei, Yan Song, Journal of Experimental Botany . 2020,第18期

机译：高粱QTGW1A编码G蛋白亚基，并充当晶粒尺寸的负调节器
2. MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava [J] . Ping’an Ma, Xin Chen, Chen Liu, Frontiers in Plant Science . 2017,第4期

机译：MeSAUR1，由一个小的Auxin-up RNA基因编码，充当转录调节剂，正调控木薯中的 ADP-葡萄糖焦磷酸化酶小亚基1a 基因。
3. Established and emerging fluorescence-based assays for G-protein function: heterotrimeric G-protein alpha subunits and regulator of G-protein signaling (RGS) proteins. [J] . Kimple RJ, Jones MB, Shutes A, Combinatorial chemistry & high throughput screening . 2003,第4期

机译：建立和新兴的基于荧光的G蛋白功能检测方法：异三聚体G蛋白α亚基和G蛋白信号传导（RGS）蛋白的调节剂。
4. Growth performance of weaner pigs fed diets containing grains milled to differentparticle sizes. I. Sorghum [C] . G. T. Nguyen, C. Collins, D. Henman, Biennial Conference of the Australasian Pig Science Association . 2015

机译：断奶猪的生长性能喂养含有谷物磨削的饮食酿造成不同的粒子尺寸。 I.高粱
5. Modulation of N-type calcium channels by G-protein betagamma subunits and regulators of G-protein signaling. [D] . Zhou, Janice Y. 2002

机译：由G蛋白betagamma亚基和G蛋白信号调节因子对N型钙通道的调节。
6. G-protein βγ subunits determine grain size through interaction with MADS-domain transcription factors in rice [O] . Qian Liu, Ruixi Han, Kun Wu, -1

机译：G蛋白βγ亚基通过与水稻中MADS结构域转录因子的相互作用决定籽粒大小
7. Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit). [O] . Dohlman, H G, Song, J, Ma, D, 1996

机译：Sst2，酵母酿酒酵母中信息素信号的负调节剂：表达，定位，遗传相互作用以及与Gpa1（G蛋白α亚基）的物理关联。
8. Determination of Grain Size Distribution Function Using Two-Dimensional Fourier Transforms of Tone Pulse Encoded Images. [R] . generazio, e. r. 1986

机译：用脉冲编码图像的二维傅里叶变换确定粒度分布函数。

Sorghum qTGW1a encodes a G-protein subunit and acts as a negative regulator of grain size

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