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首页> 外文期刊>Journal of Experimental Botany >Nitric oxide-dependent regulation of sweet pepper fruit ripening
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Nitric oxide-dependent regulation of sweet pepper fruit ripening

机译：甜椒果实成熟的一氧化氮依赖性调节

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摘要

Ripening is a complex physiological process that involves changes in reactive nitrogen and oxygen species that govern the shelf-life and quality of fruits. Nitric oxide (NO)-dependent changes in the sweet pepper fruit transcriptome were determined by treating fruits at the initial breaking point stage with NO gas. Fruits were also harvested at the immature (green) and ripe (red) stages. Fruit ripening in the absence of NO resulted in changes in the abundance of 8805 transcripts whose function could be identified. Among these, functional clusters associated with reactive oxygen/nitrogen species and lipid metabolism were significantly modified. NO treatment resulted in the differential expression of 498 genes framed within these functional categories. Biochemical analysis revealed that NO treatment resulted in changes in fatty acid profiling, glutathione and proline contents, and the extent of lipid peroxidation, as well as increases in the activity of ascorbate peroxidase and lipoxygenase. These data provide supporting evidence for the crucial role of NO in the ripening of pepper fruit.
机译：成熟是一种复杂的生理过程，涉及控制果实保质和质量的反应性氮和氧物种的变化。通过在没有气体的初始断裂点阶段处理果实测定甜椒果转录组的一氧化氮（NO）依赖性变化。还在未成熟（绿色）和成熟（红色）阶段中收获水果。在没有否的情况下，果实在没有导致的8805转录物的变化中成熟的，其功能可以识别。其中，与反应性氧/氮气和脂质代谢相关的功能簇进行了显着修饰。没有治疗导致498个基因的差异表达在这些功能类别中陷入困境。生化分析显示，没有治疗导致脂肪酸分析，谷胱甘肽和脯氨酸含量的变化以及脂质过氧化的程度，以及抗坏血酸过氧化物酶和脂氧合酶活性的增加。这些数据提供了支持证据，以便在辣椒果实成熟中的毫无疑问。

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