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Analysis of bacterial polyhydroxybutyrate production by multimodal nanoimaging

机译:多峰纳米成像分析细菌多羟基丁酸酯生产

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In this paper, we will employ two microscopy techniques, transmission electron microscopy and infrared nanospectromicroscopy, to study the production of polyhydroxybutyrate in Rhodobacter capsulatus and to evaluate the influence of glucose and acetone on the production yield. The results overlap which leads us to a consistent conclusion, highlighting that each technique brings specific and complementary information. By using electron microscopy and infrared nanospectromicroscopy we have proved that both glucose and acetone had a positive effect on the biopolymer production, although the first study done by Fourier transform infrared spectroscopy only identified the effect of acetone. In conclusion, we have now established a method to be able to perform fast diagnostic for PHB production
机译:在本文中,我们将采用透射电子显微镜和红外纳米光谱两种显微镜技术研究荚膜红球菌中多羟基丁酸酯的生产,并评估葡萄糖和丙酮对产量的影响。结果重叠,使我们得出一致的结论,突出表明每种技术都带来了特定且互补的信息。通过电子显微镜和红外纳米光谱,我们已经证明葡萄糖和丙酮对生物聚合物的生产都有积极作用,尽管通过傅立叶变换红外光谱法进行的第一项研究仅确定了丙酮的作用。总之,我们现在已经建立了一种能够对PHB生产进行快速诊断的方法

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