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Flux analysis and metabolomics for systematic metabolic engineering of microorganisms

机译：用于微生物系统代谢工程的通量分析和代谢组学

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摘要

Rational engineering of metabolism is important for bio-production using microorganisms. Metabolic design based on in silica simulations and experimental validation of the metabolic state in the engineered strain helps in accomplishing systematic metabolic engineering. Flux balance analysis (FBA) is a method for the prediction of metabolic phenotype, and many applications have been developed using FBA to design metabolic networks. Elementary mode analysis (EMA) and ensemble modeling techniques are also useful tools for in silico strain design. The metabolome and flux distribution of the metabolic pathways enable us to evaluate the metabolic state and provide useful clues to improve target productivity. Here, we reviewed several computational applications for metabolic engineering by using genome-scale metabolic models of microorganisms. We also discussed the recent progress made in the field of metabolomics and C-13-metabolic flux analysis techniques, and reviewed these applications pertaining to bio-production development. Because these in silica or experimental approaches have their respective advantages and disadvantages, the combined usage of these methods is complementary and effective for metabolic engineering
机译：合理的代谢工程对于使用微生物进行生物生产非常重要。基于二氧化硅模拟的代谢物设计和工程菌株代谢状态的实验验证有助于完成系统的代谢工程。助焊剂平衡分析（FBA）是一种预测代谢表型的方法，并且已经使用FBA开发了许多应用来设计代谢网络。基本模式分析（EMA）和整体建模技术也是计算机应变设计的有用工具。代谢途径的代谢组和通量分布使我们能够评估代谢状态，并提供有用的线索来提高目标生产力。在这里，我们回顾了使用微生物的基因组规模代谢模型进行代谢工程的一些计算应用。我们还讨论了代谢组学和C-13代谢通量分析技术领域的最新进展，并回顾了与生物生产发展有关的这些应用。由于这些在硅胶或实验方法中各有优缺点，因此这些方法的组合使用对代谢工程是互补和有效的

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