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首页> 外文期刊>Journal of feline medicine and surgery >Circulating cell-free DNA does not harbour a diagnostic benefit in cats with feline diffuse iris melanomas
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Circulating cell-free DNA does not harbour a diagnostic benefit in cats with feline diffuse iris melanomas

机译：无循环的细胞DNA不会含有猫的猫衍生物弥漫性虹膜黑色素瘤

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Objectives Feline diffuse iris melanoma (FDIM) is the most common malignant primary intraocular tumour in cats, with reported metastases rates between 19% and 63%. Currently, the only available diagnostic tool for a tentative diagnosis is histopathological examination of the enucleated eye. Therefore, the veterinary ophthalmologist is often faced with the dilemma of whether to enucleate an oftentimes visual eye or to continue monitoring, with the risk of metastases developing. In the past, cell-free DNA (cfDNA) gained more attention in human medicine, especially in the field of oncology. Prior studies have shown the use of cfDNA as diagnostic or prognostic markers in canine and human cancer patients. Therefore, the aim of this study was to investigate cfDNA concentration and integrity in cats with FDIMs compared with cats with benign iris naevi and without ocular abnormalities. Methods cfDNA from plasma of cats with iris melanoma (n = 34), iris naevus (n = 30) and without ocular abnormalities (n = 32) were extracted. Primer and probes for feline amyloid beta precursor protein ( APP ) and beta actin ( ACTB ) were designed for amplicons of various lengths and quantitative PCRs of extracted cfDNA were performed to measure cfDNA concentration and integrity of the plasma samples. Differences of cfDNA concentrations and integrity levels between the three groups (iris melanoma, iris naevi and controls) were analysed using the Mann–Whitney U-test. Results cfDNA concentration and integrity analysis revealed no significant differences between the cats with iris melanoma, iris naevus or the control group ( P >0.01). Cats with metastases showed similar cfDNA concentration and integrity to cats without metastases. Conclusions and relevance cfDNA concentration and integrity seem to be insufficient as a diagnostic or prognostic marker in cats with FDIMs.
机译：目标猫科动物弥漫性虹膜黑色素瘤（FDIM）是猫中最常见的恶性初级眼肿瘤，报道的转移率为19％和63％。目前，暂定诊断的唯一可用的诊断工具是对肌肉眼的组织病理学检查。因此，兽医眼科医生通常面临困境，无论是对视觉眼睛或继续监测的困境，具有转移的风险。在过去，无细胞DNA（CFDNA）在人类医学中获得更多关注，特别是在肿瘤学领域。先前的研究表明，使用CFDNA作为犬和人癌患者的诊断或预后标志物。因此，该研究的目的是在与良性虹膜Naevi和没有眼部异常的猫的猫相比，研究与FDIM的猫的CFDNA浓度和完整性。方法提取来自虹膜黑色素瘤（n = 34），虹膜Naevus（n = 30）的猫血浆的CFDNA，没有眼部异常（n = 32）。设计用于翅膀淀粉样蛋白β前体蛋白（APP）和β肌动蛋白（ACTB）的底漆和探针，用于进行各种长度的扩增子和提取的CFDNA的定量PCR，以测量血浆样品的CFDNA浓度和完整性。使用Mann-Whitney U-Test分析三组（虹膜黑色素瘤，虹膜Melanoma，Iris Naevi和对照之间的CFDNA浓度和完整性水平的差异。结果CFDNA浓度和完整性分析显示虹膜与虹膜黑色素瘤，虹膜Naevus或对照组之间没有显着差异（P> 0.01）。具有转移的猫表现出与没有转移的猫的相似CFDNA浓度和完整性。结论和相关性CFDNA浓度和完整性似乎不足以作为FDIMS猫的诊断或预后标志物。

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