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Recombinant protein secretion in Escherichia coli.

机译:大肠杆菌中的重组蛋白分泌。

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摘要

The secretory production of recombinant proteins by the Gram-negative bacterium Escherichia coli has several advantages over intracellular production as inclusion bodies. In most cases, targeting protein to the periplasmic space or to the culture medium facilitates downstream processing, folding, and in vivo stability, enabling the production of soluble and biologically active proteins at a reduced process cost. This review presents several strategies that can be used for recombinant protein secretion in E. coli and discusses their advantages and limitations depending on the characteristics of the target protein to be produced.
机译:革兰氏阴性细菌大肠埃希氏菌分泌产生重组蛋白比作为包涵体的细胞内产生具有多个优势。在大多数情况下,将蛋白质靶向周质空间或培养基有助于下游加工,折叠和体内稳定性,从而能够以降低的加工成本生产可溶性和生物活性蛋白质。这篇综述提出了可用于在大肠杆菌中分泌重组蛋白的几种策略,并讨论了它们的优点和局限性,具体取决于要生产的靶蛋白的特性。

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