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Bioprocessing strategies to improve heterologous protein production in filamentous fungal fermentations.

机译:改善丝状真菌发酵中异源蛋白质生产的生物加工策略。

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摘要

Filamentous fungi have long been used for the production of metabolites and enzymes. With developments in genetic engineering and molecular biology, filamentous fungi have also achieved increased attention as hosts for recombinant DNA. However, the production levels of non-fungal proteins are usually low. Despite the achievements obtained using molecular tools, the heterologous protein loss caused by extracellular fungal protease degradation persists. This review provides an overview of the potential bioprocessing strategies that can be applied to inhibit protease activity thereby enhancing heterologous protein production.
机译:丝状真菌长期以来一直用于产生代谢产物和酶。随着基因工程和分子生物学的发展,丝状真菌作为重组DNA的宿主也引起了越来越多的关注。但是,非真菌蛋白的生产水平通常较低。尽管使用分子工具取得了成就,但由于细胞外真菌蛋白酶降解引起的异源蛋白质损失仍然存在。这篇综述概述了可用于抑制蛋白酶活性从而增强异源蛋白质生产的潜在生物加工策略。

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