Prediction of protein retention in hydrophobic interaction chromatography.

Mahn A; Asenjo JA

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Prediction of protein retention in hydrophobic interaction chromatography.

机译：疏水相互作用色谱中蛋白质保留的预测。

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Hydrophobic interaction chromatography (HIC) is a powerful technique for protein separation. This review examines methodologies for predicting protein retention time in HIC involving elution with salt gradients. The methodologies discussed consider three-dimensional structure data of the protein and its surface hydrophobicity. Despite their limitations, the methods discussed are useful in designing purification processes for proteins and easing the tedious experimental work that is currently required for developing purification protocols.

机译：疏水相互作用色谱（HIC）是一种用于蛋白质分离的强大技术。这篇综述检查了预测HIC中蛋白质保留时间的方法，包括用盐梯度洗脱。所讨论的方法考虑了蛋白质的三维结构数据及其表面疏水性。尽管有其局限性，但所讨论的方法可用于设计蛋白质的纯化过程并简化开发纯化方案目前所需的繁琐实验工作。

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《Biotechnology Advances: An International Review Journal》 |2005年第5期|共10页
作者
Mahn A; Asenjo JA;
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正文语种 eng
中图分类基础医学;
关键词
Chromatography; Models; Chemical; Protein Interaction Mapping; Proteins; 色谱法; 模型; 化学; 蛋白质类; 溶剂; 水;

机译：Chromatography;Models;Chemical;Protein Interaction Mapping;Proteins;色谱法;模型;化学;蛋白质类;溶剂;水;

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1. Prediction of protein retention in hydrophobic interaction chromatography. [J] . Mahn A, Asenjo JA Biotechnology Advances: An International Review Journal . 2005,第5期

机译：疏水相互作用色谱中蛋白质保留的预测。
2. Prediction of retention times of proteins in hydrophobic interaction chromatography using only their amino acid composition [J] . Salgado JC, Rapaport I, Asenjo JA Journal of chromatography, A: Including electrophoresis and other separation methods . 2005,第1a2期

机译：仅使用氨基酸组成预测疏水相互作用色谱中蛋白质的保留时间
3. Prediction of protein retention times in hydrophobic interaction chromatography by robust statistical characterization of their atomic-level surface properties [J] . Hanke Alexander T., Klijn Marieke E., Verhaert Peter D. E. M., Biotechnology Progress . 2016,第2期

机译：通过其原子级表面特性的可靠统计表征预测疏水相互作用色谱中的蛋白质保留时间
4. Proteomics approach for predicting retention behavior of a mixture of proteins during hydrophobic interaction chromatography [C] . Ryan Swanson, Charles E. Glatz Annual biochemical engineering symposium . 2012

机译：蛋白质组学方法在疏水相互作用色谱法中预测蛋白质混合物的保留行为
5. Application of a Stoichiometric Water Displacement Model and Property-Retention Relationships to Adsorption and Unfolding of Proteins in Hydrophobic Interaction Chromatography. [D] . Deitcher, Robert William, Jr. 2010

机译：化学计量水置换模型和性质保留关系在疏水相互作用色谱中蛋白质吸附和解折叠中的应用。
6. Ca2+-dependent hydrophobic-interaction chromatography. Isolation of a novel Ca2+-binding protein and protein kinase C from bovine brain. [O] . M P Walsh, K A Valentine, P K Ngai, 1984

机译：Ca2 +依赖性疏水相互作用色谱。从牛脑中分离出新型的Ca2 +结合蛋白和蛋白激酶C。
7. Ca2+-dependent hydrophobic-interaction chromatography. Isolation of a novel Ca2+-binding protein and protein kinase C from bovine brain. [O] . Walsh, M P, Valentine, K A, Ngai, P K, 1984

机译：Ca2 +依赖性疏水相互作用色谱。从牛脑中分离出新型的Ca2 +结合蛋白和蛋白激酶C。

Prediction of protein retention in hydrophobic interaction chromatography.

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