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首页> 外文期刊>Journal of Food Science and Technology >Development of amperometric biosensor in modified carbon paste with enzymatic preparation based on lactase immobilized on carbon nanotubes
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Development of amperometric biosensor in modified carbon paste with enzymatic preparation based on lactase immobilized on carbon nanotubes

机译：基于碳纳米管固定在碳纳米管上固定的乳糖酶的改性碳浆料中碳浆料中的化工浆料的发展

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The variety of products derived from milk, without or with lactose, encourages the development of more effective analytical techniques that can be applied to the quality control of both the production line and the final products. Thus, in this work an efficient and minimally invasive method for the detection of lactose was proposed, using a biosensor containing the enzyme lactase (LAC) immobilised on carbon nanotubes (CNTs) that, when reacting with lactose, emit an electrochemical signal. This biosensor was connected to a potentiostat, and its electrochemical cell was composed of the following three electrodes: reference electrode (Ag/AgCl), auxiliary electrode (platinum wire), and working electrode (biosensor) on which graphite (carbon) paste (CP), CNTs, and LAC were deposited. The transmission electron microscopy and scanning electron microscopy were used in the characterisation of the composite morphology, indicating excellent interactions between the CNTs and LAC. The sensitivity of the CP/LAC/CNT biosensor was determined as 5.67 mu A cm(-2).mmol(-1) L and detection limits around 100 x 10(-6) mol L-1 (electrode area = 0.12 cm(2)) and an increase in the stability of the system was observed with the introduction of CNTs because, with about 12 h of use, there was no variation in the signal (current). The results indicate that the association between the CNTs and LAC favoured the electrochemical system.
机译：源自牛奶，没有或乳糖的产品，鼓励开发更有效的分析技术，可以应用于生产线和最终产品的质量控制。因此，在该工作中，提出了一种用于检测乳糖的有效和微创方法，使用含有固定在碳纳米管（CNT）上的酶乳糖酶（LAC）的生物传感器，即当与乳糖反应时，发出电化学信号。该生物传感器连接到恒温器，其电化学电池由以下三电极组成：参考电极（Ag / AgCl），辅助电极（铂丝）和石墨（碳）浆料的工作电极（生物传感器）（CP ），CNT和Lac被沉积。透射电子显微镜和扫描电子显微镜用于复合形态的表征，表明CNT和LAC之间的优异相互作用。 CP / LAC / CNT生物传感器的敏感性确定为5.67μmMmol（-2）.mmol（-1）L和约100×10（-6）摩尔L-1的检测限（电极面积= 0.12cm（ 2））通过引入CNT而观察到系统稳定性的增加，因为，使用约12小时，信号（电流）没有变化。结果表明，CNT和LAC之间的关联赞成电化学系统。

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