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首页> 外文期刊>Journal of genetics >Molecular cytogenetic characterization of a new wheat Secale africanum 2R ~a(2D) substitution line for resistance to stripe rust
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Molecular cytogenetic characterization of a new wheat Secale africanum 2R ~a(2D) substitution line for resistance to stripe rust

机译：用于抗条纹锈病的新小麦分离非洲2R〜A（2D）替代线的分子细胞遗传学表征

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摘要

A stable, highly fertile wheat Secale africanum substitution line LF24, derived from the F _7 generation of a cross between Mianyang11 (MY11) and Triticum durum, S. africanum amphiploid (YF) was identified through molecular cytogenetic analysis. Application of C-banding, in situ hybridization and molecular markers analysis showed that LF24 was a wheat S. africanum 2R ~a(2D) substitution line. When inoculated with stripe rust isolates, T. durum and MY11 were highly susceptible, while S. africanum, YF and LF24 were immune. It is confirmed through molecular cytogenetic analysis that the stripe rust resistance of LF24 was derived from S. africanum chromosome 2R ~a. We compared the banding patterns and disease resistance of reported chromosomes 2R from different S. cereale introduced into wheat background, and found that there was new stripe rust resistance gene(s) on S. africanum 2R ~a. LF24 is a new substitution line which can be used as stripe rust resistant source in wheat improvement.
机译：通过分子细胞遗传学分析鉴定了源自绵阳11（MY11）和Triticum，S. Africoanum Amphiploid（YF）之间的F _7生成的稳定，高度肥沃的小麦分检。 C型杂交的应用，原位杂交和分子标记分析表明，LF24是小麦S.非洲2R〜A（2D）替代线。 当用条纹防锈分离物接种时，T.Durum和My11非常敏感，而S. Africanum，YF和LF24则免疫。 通过分子细胞遗传学分析确认，即LF24的条纹耐抗抗性来自S. Africanum染色体2R〜a。 我们将报告的染色体2R从不同的S. CEREALE引入小麦背景中的染色体2R的疾病抗性和抗病抗性，发现S. AFRORANUM 2R〜a上存在新的条纹锈蚀基因。 LF24是一种新的替代线，可以用作小麦改善的条纹防锈源。

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  • 来源
    《Journal of genetics》 |2011年第2期|共5页
  • 作者

    Lei M.; Li G.; Zhang S.; Liu C.; Yang Z.;

  • 作者单位

    School of Life Science and Technology University of Electronic Science and Technology of China 4 Jianshe Road Chengdu 610054 China;

    School of Life Science and Technology University of Electronic Science and Technology of China 4 Jianshe Road Chengdu 610054 China;

    School of Life Science and Technology University of Electronic Science and Technology of China 4 Jianshe Road Chengdu 610054 China;

    School of Life Science and Technology University of Electronic Science and Technology of China 4 Jianshe Road Chengdu 610054 China;

    School of Life Science and Technology University of Electronic Science and Technology of China 4 Jianshe Road Chengdu 610054 China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    C-banding; in situ hybridization; Secale africanum; stripe rust;

    机译：C-绑定;原位杂交;Secale Africanum;条纹生锈;

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