...
首页> 外文期刊>Journal of genetics >A universal, rapid, and inexpensive method for genomic DNA isolation from the whole blood of mammals and birds
【24h】

A universal, rapid, and inexpensive method for genomic DNA isolation from the whole blood of mammals and birds

机译:来自哺乳动物和鸟类全血的基因组DNA分离的普遍,快速,廉价的方法

获取原文
获取原文并翻译 | 示例
           

摘要

There is no 'one' procedure for extracting DNA from the whole blood of both mammals and birds, since each species has a unique property that require different methods to release its own DNA. Therefore, to obtain genomic DNA, a universal, rapid, and noncostly method was developed. A very simple biological basis is followed in this procedure, in which, when the blood is placed in water, it rapidly enters the RBCs by osmosis and causes cells to burst by hemolysis. The validity of extracting genomic DNA was confirmed by several molecular biological experiments. It was found that this method provides an efficient and versatile alternative for extracting bulk amounts of highly-qualified DNA from the blood of a wide range of species. This is the first manuscript that describes use of distilled water as the only eliminator of RBCs among all other known DNA extraction techniques.
机译:从哺乳动物和鸟类的全血中提取DNA没有“一个”程序,因为每种物种具有不同的性质,需要不同的方法来释放其自己的DNA。 因此,为了获得 基因组DNA ,通用 ,快速, noncostly 方法 被开发 。 在该过程中遵循非常简单的生物基础,其中,当血液置于水中时,它通过渗透迅速进入RBC并使细胞通过溶血爆发。 提取 基因组DNA 的有效性 是由几个 分子生物学 实验所证实。 发现该方法提供了一种高效且多功能的替代方案,用于从各种物种的血液中提取大量高合格的DNA。 这是第一个描述蒸馏水作为所有其他已知的DNA提取技术中唯一的RBC的消除器的使用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号