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首页> 外文期刊>Journal of genetics >Cooverexpression of EpCAM and c-myc genes in malignant breast tumours
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Cooverexpression of EpCAM and c-myc genes in malignant breast tumours

机译：对恶性乳腺肿瘤中EPCAM和C-MYC基因的辅导印迹

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摘要

The overexpression of epithelial cell adhesion molecule (EpCAM), a proto-oncogene, affects progression, treatment, and diagnosis of many adenocarcinomas. C-myc has been shown to be a downstream target of EpCAM and is also one of the most important proto-oncogenes routinely overexpressed in breast cancer. However, cooverexpression of EpCAM and c-myc genes has not been investigated in breast cancer tissues, particularly in Iranian population. The aim of this study was to assess the expression of EpCAM and c-myc genes in malignant breast cancer tissues using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) followed by analyses of the association between the outcomes. In this study, 122 fresh tissues, including 104 malignant and 18 benign samples, were disrupted by mortar and pestle, and then the RNA was isolated from the samples and converted to cDNA. The relative expression levels of EpCAM and c-myc genes were measured by 2 (-Delta Delta Ct) method using RT-qPCR. EpCAM protein level was also assessed in 66 cases using Western blot technique. Using RT-qPCR method, our results showed that EpCAM was overexpressed in 48% of malignant and 11.1% of benign samples. Evaluating EpCAM protein overexpression in a portion of samples depicted the fully concordance rate between Western blot and RT-qPCR techniques. C-myc expression was first evaluated by RT-qPCR method, showing the overexpression rate of 39% and 28% in malignant and benign samples, respectively. These data were also quite concordant with the clinically available immunohistochemistry reports of the same samples studied in this study. Importantly, overexpression of EpCAM and c-myc was significantly associated and showed an agreement of 57.3%. This study demonstrated the cooverexpression of EpCAM and c-myc in breast tumours collected from breast cancer patients of the Iranian population. EpCAM and c-myc positive cases were significantly associated with reduced and enhanced risk of ER/PR positivity respectively. However, both were associated with grade III of breast cancer.
机译：上皮细胞粘附分子（EPCAM）的过表达，一种原癌基因，影响许多腺癌的进展，治疗和诊断。 C-MYC已被证明是EPCAM的下游靶标，也是乳腺癌中常规过度表达的最重要的原癌基因之一。然而，乳腺癌组织中尚未研究EPCAM和C-MYC基因的辅导印迹，特别是在伊朗人口中。本研究的目的是利用逆转录酶定量聚合酶链反应（RT-QPCR）评估对恶性乳腺癌组织中的EPCAM和C-MYC基因的表达，然后分析结果之间的关联。在本研究中，由砂浆和杵中断122个新鲜组织，其中包括104个恶性和18个良性样品，然后从样品中分离RNA并转化为cDNA。使用RT-QPCR的2（-Delta Delta CT）方法测量EPCAM和C-MYC基因的相对表达水平。在66例使用Western印迹技术中也评估了EPCAM蛋白质水平。使用RT-QPCR方法，我们的结果表明，EPCAM在48％恶性和11.1％的良性样品中过表达。在一部分样品中评估EPCAM蛋白过表达，描绘了蛋白质印迹和RT-QPCR技术之间的完全一致性率。首先通过RT-QPCR方法评估C-MYC表达，分别显示出恶性和良性样品的过表达率为39％和28％。这些数据也与本研究中研究的相同样本的临床可用免疫组织化学报告相当合作。重要的是，EPCAM和C-MYC的过度表达显着相关，并显示了57.3％的协议。本研究表明，从伊朗人口乳腺癌患者收集的乳腺癌中EPCAM和C-MYC的辅导印迹。 EPCAM和C-MYC阳性病例分别与ER / PR阳性的风险显着相关。然而，两者都与乳腺癌的级别有关。

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