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首页> 外文期刊>Journal of genetics >Molecular characterization, expression profile of the FSHR gene and its association with egg production traits in muscovy duck
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Molecular characterization, expression profile of the FSHR gene and its association with egg production traits in muscovy duck

机译：分子表征，FSHR基因的表达谱及其与卵鸭卵产物的关联

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摘要

Follicle-stimulating hormone (FSH) and its receptor play a key role in the follicular development and regulation of steroidogenesis in the ovary and spermatogenesis in the testis. The purpose of this study was to characterize the muscovy duck FSHR gene, identify SNPs and their association with egg production traits in muscovy ducks. Here, we cloned the complementary DNA (cDNA) sequence of FSHR, and examined the expression patterns of FSHR gene in adult female muscovy duck tissues. The cloned cDNA of the muscovy duck FSHR gene shared high similarity to those of pekin duck (Anas platyrhynchos) (95.7%) and chicken (93.2%). Three different muscovy duck FSHR transcripts were identified. Quantitative real-time PCR (RT-qPCR) results showed that the FSHR gene was expressed in all the 14 tested tissues, and the highest expression level was seen in the ovary. A total of 16 SNPs were identified, among which, four SNPs were located in the coding region of FSHR. The SNP C320T is significantly associated with egg production at 59 weeks of age (), whereas the SNP A227G is significantly associated with age at first egg stage (). These results suggest that the two SNPs (A227G and C320T) of FSHR gene are associated with egg production traits and could be potential markers that can be used for marker-assisted selection programmes to increase egg production in muscovy duck.
机译：卵泡刺激激素（FSH）及其受体在睾丸中卵巢和精子发生中的卵泡开发和调节中发挥着关键作用。本研究的目的是表征番鸭FSHR基因，鉴定SNP和它们与卵鸭子的鸡蛋生产性状的关联。在这里，我们克隆了FSHR的互补DNA（cDNA）序列，并检查了成年女性Muscovy鸭组织中FSHR基因的表达模式。克隆的鸭鸭FSHR基因的cDNA与皮鸭（ANAs platyrhynchos）（95.7％）和鸡（93.2％）共同相似之处。确定了三种不同的番荔枝FSHR转录物。定量实时PCR（RT-QPCR）结果表明，在所有14个测试组织中表达FSHR基因，在卵巢中看到最高表达水平。鉴定了总共16个SNP，其中4个SNP位于FSHR的编码区域中。 SNP C320T在年龄（）的59周内与鸡蛋产生显着相关，而SNP A227G在第一个蛋阶段（）的年龄明显相关。这些结果表明，FSHR基因的两个SNP（A227G和C320T）与卵产物相关有关，并且可以是可用于标记辅助选择计划的潜在标志，以提高卵鸭中的鸡蛋生产。

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  • 来源
    《Journal of genetics》 |2017年第2期|共11页
  • 作者

    Xu Jiguo; Gao Xinfeng; Li Xing; Ye Qiao; Jebessa Endashaw; Abdalla Bahareldin Ali; Nie Qinghua;

  • 作者单位

    South China Agr Univ Coll Anim Sci Natl Local Joint Engn Res Ctr Livestock Breeding Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Coll Anim Sci Natl Local Joint Engn Res Ctr Livestock Breeding Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Coll Anim Sci Natl Local Joint Engn Res Ctr Livestock Breeding Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Coll Anim Sci Natl Local Joint Engn Res Ctr Livestock Breeding Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Coll Anim Sci Natl Local Joint Engn Res Ctr Livestock Breeding Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Coll Anim Sci Natl Local Joint Engn Res Ctr Livestock Breeding Guangzhou 510642 Guangdong Peoples R China;

    South China Agr Univ Coll Anim Sci Natl Local Joint Engn Res Ctr Livestock Breeding Guangzhou 510642 Guangdong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    FSHR gene; variant transcripts; gene expression; muscovy duck; egg production traits;

    机译：FSHR基因;变体转录物;基因表达;番鸭;鸡蛋生产性状;

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