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首页> 外文期刊>Journal of genetics >Analysis of polymorphisms and selective pressures on ama1 gene in Plasmodium knowlesi isolates from Sabah, Malaysia
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Analysis of polymorphisms and selective pressures on ama1 gene in Plasmodium knowlesi isolates from Sabah, Malaysia

机译：从马来西亚沙巴疟原虫近似疟原虫植物中AMA1基因的多态性和选择性压力分析

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摘要

The apical membrane antigen-1 (AMA-1) of Plasmodium spp. is a merozoite surface antigen that is essential for the recognition and invasion of erythrocytes. Polymorphisms occurring in this surface antigen will cause major obstacles in developing effective malaria vaccines based on AMA-1. The objective of this study was to characterize ama1 gene in Plasmodium knowlesi isolates from Sabah. DNA was extracted from blood samples collected from Keningau, Kota Kinabalu and Kudat. The Pkama-1 gene was amplified using nested PCR and subjected to bidirectional sequencing. Analysis of DNA sequence revealed that most of the nucleotide polymorphisms were synonymous and concentrated in domain I of PkAMA-1. Forteen haplotypes were identified based on amino acid variations and haplotype K5 was the most common haplotype. d(N)/d(S) ratios implied that purifying selection was prevalent in Pkama1 gene. Fu and Li's D and F values further provided evidence of negative selection acting on domain II of Pkama1. Low nucleotide diversity was also detected for the Pkama1 sequences, which is similar to reports on Pkama1 from Peninsular Malaysia and Sarawak. The presence of purifying selection and low nucleotide diversity indicated that domain II of Pkama1 can be used as a target for vaccine development.
机译：疟原虫SPP的顶端膜抗原-1（AMA-1）。是一个Merozoite表面抗原，对于识别和侵袭红细胞是必不可少的。在该表面抗原中发生的多态性将导致基于AMA-1的有效疟疾疫苗产生重大障碍。本研究的目的是将AMA1基因在Sabah的疟原虫中的疟原虫分离。从来自Kenenau，Kota Kinababalu和Kudat收集的血液样本中提取DNA。使用巢式PCR扩增pKama-1基因并进行双向测序。 DNA序列的分析显示，大多数核苷酸多态性是同义词和浓缩在Pkama-1的结构域I中。基于氨基酸变化和单倍型K5鉴定了Forteen Haplotypes是最常见的单倍型。 D（n）/ d（s）比例暗示纯化选择在pkama1基因中普遍。傅和李的D和F值进一步提供了对PKAMA1结构域II作用的负面选择的证据。对于pkama1序列，也检测到低核苷酸多样性，这类似于来自半岛马来西亚和沙捞越的Pkama1的报道。纯化选择和低核苷酸多样性的存在表明Pκ1的结构域II可以用作疫苗发育的靶标。

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