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首页> 外文期刊>Journal of genetics >Marker-assisted introgression of opaque2 allele for rapid conversion of elite hybrids into quality protein maize
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Marker-assisted introgression of opaque2 allele for rapid conversion of elite hybrids into quality protein maize

机译：Opaque2等位基因的标记辅助血栓增强精英杂交种进入优质蛋白玉米

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摘要

Maize is a valuable source of food and feed worldwide. Maize endosperm protein is, however nutritionally poor due to the reduced levels of two essential amino acids, lysine and tryptophan. In this study, recessive opaque2 (o2) allele that confers enhanced endosperm lysine and tryptophan, was introgressed using marker-assisted backcross breeding into three normal inbred lines (HKI323, HKI1105 and HKI1128). These are the parental lines of three popular medium-maturing single cross hybrids (HM4, HM8 and HM9) in India. Gene-based simple sequence repeat (SSR) markers (umc1066 and phi057) were successfully deployed for introgression of o2 allele. Background selection using genome-based SSRs helped in recovering of recurrent parent genome. The newly developed quality protein maize (QPM) inbreds showed modified kernels (25-50% opaqueness) coupled with high degree of phenotypic resemblance to the respective recipient lines, including grain yield. In addition, endosperm protein quality showed increased lysine and tryptophan in the inbreds to the range of 52-95% and 47-118%, respectively. The reconstituted QPM hybrids recorded significant enhancement of endosperm lysine (48-74%) and tryptophan (55-100%) in the endosperm. The QPM hybrids exhibited high phenotypic similarity with the original hybrids for morphological and yield contributing traits along with responses to some major diseases like turcicum leaf blight and maydis leaf blight. The grain yield of QPM hybrids was at par with their original versions under multilocation testing. These elite, high-yielding QPM hybrids with improved protein quality have been released and notified for commercial cultivation, and hold significant promise for improving nutritional security.
机译：玉米是全世界的宝贵食物来源。然而，由于两个必需氨基酸，赖氨酸和色氨酸的水平降低，玉米胚乳蛋白是营养较差的。在本研究中，使用标记辅助回复繁殖分为三种正常自交系（HKI323，HKI1105和HKI1128），赋予增强的胚乳赖氨酸和色氨酸的隐性OPAQue2（O2）等位基因血糖均多血糖血糖均匀。这些是印度三种流行的中等成熟的单一交叉杂交种（HM4，HM8和HM9）的父母。基于基于基因的简单序列重复（SSR）标记（SSR）标记（UMC1066和PHI057）被成功部署用于O2等位基因的迟钝。使用基于基于基于基于基于基于基于基于基于基于基于基于基于基于基于基于基于基于基于基于基于父母基因组的SSR。新开发的质量蛋白质玉米（QPM）近铬显示出改性核（25-50％的不透明性），其与各自的受体线具有高度表型相似性，包括谷物产量。此外，胚乳蛋白质质量均显示出近红细胞中的赖氨酸和色氨酸，分别为52-95％和47-118％。重构的QPM杂种物记录了胚乳液（48-74％）和色氨酸（55-100％）的显着增强。 QPM杂种与原始杂交种表现出高表型相似性，用于对形态和产量有贡献性状的性状，以及对草原叶片枯萎病和Maydis Leaf枯萎病的一些主要疾病的反应。 QPM杂交种的谷物产量与其原始版本相提并论。这些精英，高产QPM杂交种具有改善的蛋白质质量并通知商业培养，并对提高营养安全性具有重要的承担。

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