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首页> 外文期刊>Journal of genetics >An improved microsatellite panel to assess genetic variability of the Italian smooth newt (Lissotriton vulgaris meridionalis)
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An improved microsatellite panel to assess genetic variability of the Italian smooth newt (Lissotriton vulgaris meridionalis)

机译：一种改进的微卫星面板，以评估意大利光滑蝾螈的遗传变异（Lissotriton Vulgaris Meridionalis）

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摘要

Habitat loss and fragmentation are threatening amphibians by increasing population isolation. However, artificial waterbodies created for livestock may contrast this phenomenon by providing surrogate habitats for amphibians. Here, we performed a genetic study on an amphibian species, Lissotriton vulgaris nieridionalis, in a rural area in central Italy where natural wetlands are disappearing and drinking troughs for cattle watering are widespread. Specifically, we tested a panel of microsatellite markers to identify a suitable tool for addressing conservation genetic issues of this species that is undergoing severe local decline. Twelve of the 20 tested loci produced reliable amplifications and were polymorphic. Three distinct units with a low level of gene flow were distinguished and the population genetic structuring overlapped with geographic distribution. Such loci will be useful to assess the genetic diversity of the species across multiscale levels for its management and conservation.
机译：栖息地损失和碎片是通过增加人口孤立来威胁两栖动物。然而，为牲畜创造的人造水域可以通过为两栖动物提供替代栖息地来对比这种现象。在这里，我们对意大利中部地区的农村地区进行了遗传研究，在一个农村地区，天然湿地消失，牛水饮水的饮酒是普遍的。具体而言，我们测试了一个微卫星标记的面板，以鉴定用于解决经历严重的当地下降的物种的保护遗传问题的合适工具。 12个测试基因座的12个产生可靠的扩增，并且是多态的。区分具有低基因流量水平的三个不同单位，并且群体遗传结构与地理分布重叠。这种基因座可用于评估多尺度水平的物种的遗传多样性，以获得其管理和保护。

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