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首页> 外文期刊>Journal of genetics >Novel alternatively spliced isoforms of MEF2A and their mRNA expression patterns in pigs
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Novel alternatively spliced isoforms of MEF2A and their mRNA expression patterns in pigs

机译：新颖的猪肉和它们的mRNA表达模式可选择剪接同种型

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摘要

The present study aimed to identify the alternatively spliced isoforms of pig MEF2A gene and to determine theirmRNA expression patterns. Four alternatively spliced isoforms of pig MEF2A gene (i.e. MEF2A1, MEF2A2, MEF2A3 and MEF2A4) were cloned according to the results of transcriptome sequencing. The fifth to eighth exons of MEF2A1were normally spliced. In MEF2A2, the fifth exon was missing; the sixth exon had an extra 138 bp at its 5' end, and the seventh exon had an extra 102 bp at its 3' end. In MEF2A3, the fifth exon was missing, and the sixth exon had an additional 138 bp at its 5' end. In MEF2A4, the seventh exon had an extra 102 bp at its 3' end. Quantitative real-time polymerase chain reaction (qPCR) analysis indicated that the expression profiles of the four alternatively spliced transcripts in the longissimus dorsi differed between the Mashen and Large White pigs. MEF2A1 and MEF2A2 expression levels were the highest at 90 days of age and lowest at 180 days of age. MEF2A3 and MEF2A4 expression levels increased with age (in days). The four alternatively spliced isoforms of MEF2A were also expressed in the small intestine, cerebellum, pancreas, heart and lung. The discovery of new alternatively spliced transcripts of the MEF2A gene may be utilized in understanding its biological functions.
机译：本研究旨在鉴定猪MEF2A基因的可替代的同种型，并确定其患有它们的表达模式。根据转录组测序的结果，克隆了四种猪MeF2a基因的猪MeF2a基因的异构型（即MeF2a1，MeF2a2，mef2a3和mef2a4）。通常拼接的MEF2A1的第五个至第八个外显子。在MEF2A2中，缺少第五个外显子;第六个外显子在其5'结束时有一个额外的138年，第七个外显子在其3'结束时额外有102磅。在MEF2A3中，第五个外显着缺失，第六个外显子在其5'末端额外有138磅。在MEF2A4中，第七个外显子在其3'末端有一个额外的102bp。定量实时聚合酶链反应（QPCR）分析表明，在马须和大白猪之间的4个交替剪接转录物中的四个也是剪接转录物的表达谱不同。 MeF2A1和MEF2A2表达水平在90天的年龄最高，最低为180天。 MEF2A3和MEF2A4表达水平随年龄（以天）增加。 MEF2a的四种也是剪接的同种型也在小肠，小脑，胰腺，心脏和肺中表达。可以利用MeF2A基因的新的可选转录转录物的发现，以了解其生物学功能。

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  • 来源
    《Journal of genetics》 |2018年第4期|共9页
  • 作者

    Guo Xiao Hong; Zhang Qi; Li Meng; Gao Peng Fei; Cao Guo Qing; Cheng Zhi Min; Zhang Ning Fang; Le Bao Yu; Liu Jian Feng; Liu Xiao Jun; Li Bu Gao;

  • 作者单位

    Shanxi Agr Univ Coll Anim Sci &

    Technol Taigu 030801 Shanxi Peoples R China;

    Shanxi Agr Univ Coll Anim Sci &

    Technol Taigu 030801 Shanxi Peoples R China;

    Shanxi Agr Univ Coll Anim Sci &

    Technol Taigu 030801 Shanxi Peoples R China;

    Shanxi Agr Univ Coll Anim Sci &

    Technol Taigu 030801 Shanxi Peoples R China;

    Shanxi Agr Univ Coll Anim Sci &

    Technol Taigu 030801 Shanxi Peoples R China;

    Shanxi Agr Univ Coll Anim Sci &

    Technol Taigu 030801 Shanxi Peoples R China;

    Shanxi Agr Univ Coll Anim Sci &

    Technol Taigu 030801 Shanxi Peoples R China;

    Shanxi Agr Univ Coll Anim Sci &

    Technol Taigu 030801 Shanxi Peoples R China;

    China Agr Univ Key Lab Anim Genet Breeding &

    Reprod Minist Agr Coll Anim Sci &

    Technol Beijing 100193 Peoples R China;

    Henan Agr Univ Coll Anim Sci &

    Vet Med Zhengzhou 450002 Henan Peoples R China;

    Shanxi Agr Univ Coll Anim Sci &

    Technol Taigu 030801 Shanxi Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    pig; MEF2A gene; alternative splicing; mRNA expression;

    机译：猪;mef2a基因;替代剪接;mRNA表达;

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