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首页> 外文期刊>Journal of genetics >Deciphering species relationships and evolution in Chenopodium through sequence variations in nuclear internal transcribed spacer region and amplified fragment-length polymorphism in nuclear DNA
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Deciphering species relationships and evolution in Chenopodium through sequence variations in nuclear internal transcribed spacer region and amplified fragment-length polymorphism in nuclear DNA

机译:通过核内转录间隔区的序列变化和核DNA中扩增的片段长度多态性通过序列变化来解入物种关系和进化

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摘要

Evaluation of sequence variations in the internal transcribed spacer (ITS) region of 19 accessions, comprising of 11 accessions of Chenopodium quinoa, eight accessions of Chenopodium album and 165 retrieved sequences of different species of Chenopodium belonging to subfamily Chenopodioideae revealed a higher intraspecific genetic diversity in Himalayan C. album than that in C. quinoa. ITS and amplified fragment-length profiles of the accessions suggest the existence of accessions of Himalayan C. album as heteromorphs of the same species rather than a heterogenous assemblage of taxa.While the evolutionary relationship reconstructed fromvariations in 184 sequences of ITS region fromspecies belonging toChenopodiaceae, Amaranthaceae, Polygonaceae and Nelumbonaceae established a paraphyletic evolution of family Chenopodiaceae, it also revealed a monophyletic evolution of Chenopodieae I. The reconstruction also established five independent lineages of the subfamily Chenopodioideae with C. album as a sister clade of C. quinoa within the tribe Chenopodieae I. The results also indicate a much younger age for Himalayan chenopods (C. album) than the reported crown age of Chenopodieae I.
机译:评价19种的内部转录间隔物(其)区域的序列变化,包括11种奎松藜藜藜,八个摘录的辛博博喹喔唑和165个属于亚家族脑内脑内核苷酸的不同种类的异丙醇的序列揭示了较高的内部遗传多样性喜马拉雅C.专辑比C.奎奴亚藜。其和扩增的碎片长度剖视图的载体建议将喜马拉雅C.专辑作为相同物种的异常存在而不是异征的异常的异常。当其区域的184个地区的序列中重建的进化关系重建, Amaranthaceae,Polygonaceae和Nelumbonaceae建立了家庭脑内核糖岩的仿真性演变,也揭示了Chenopodieae I的单味性演变。该重建还与C.藜尼亚群岛C.奎奴亚藜的C. Quinoa的C.奎奴亚州的C.奎奴亚州的孤立者群岛的五个独立谱系I.结果还表明喜马拉雅·切面板(C.专辑)比报告的Chenopodieae I.的年龄更年轻。

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