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首页> 外文期刊>Biotechnology Progress >Effect of Moderate Electric Field Frequency on Growth Kinetics and Metabolic Activity of Lactobacillus acidophilus
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Effect of Moderate Electric Field Frequency on Growth Kinetics and Metabolic Activity of Lactobacillus acidophilus

机译：适度电场频率对嗜酸乳杆菌生长动力学和代谢活性的影响

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摘要

Moderate electric fields(MEF)have been previously shown to alter the metabolic activity of microbial cells;thus,the effect of frequency and electric field would be of considerable interest.We investigated herein the effects of MEF frequency on microbial growth kinetics and bacteriocin(Lacidin A)production of Lactobacillus acidophilus OSU 133 during fermentation.The following fermentation treatments were compared:conventional(for 40 h),MEF(1V cm~(-1),for 40 h),combination of MEF(1 V cm"1,for the first 5 h)and conventional(for 35 h)at various frequency levels(45,60,and 90 Hz)all at 30 °C,and control(conventional)fermentation at 37 °C.MEF treatments with purely sinusoidal waveforms at all frequencies at 30 °C produced a shorter lag phase than conventional fermentation.However,no lag phase reduction was found for a 60 Hz waveform that contained high-frequency harmonics.There was,however,a significant increase in the bacteriocin production under early MEF treatment at 60 Hz with high-frequency harmonics.On the basis of these observations,the fermentation process is accelerated by applying pure sinusoidal MEF at the early stage of growth while a significant increase in the bacteriocin production occurs when sinusoidal field at 60 Hz with harmonics is applied at the early stage of the growth.
机译：以前已经证明了中等强度的电场（MEF）会改变微生物细胞的代谢活性;因此，频率和电场的影响将引起人们极大的兴趣。我们在此研究了MEF频率对微生物生长动力学和细菌素（Lacidin）的影响A）发酵过程中嗜酸乳杆菌OSU 133的产生。比较了以下发酵处理方法：常规（40 h），MEF（1V cm〜（-1），40 h），MEF（1 V cm“ 1，在30°C下以不同的频率水平（45,60和90 Hz）进行前5 h）和常规（35 h）的控制，并在37°C下控制（常规）发酵.MEF处理采用纯正弦波形在30°C的所有频率下产生的滞后阶段都比常规发酵短。但是，对于包含高频谐波的60 Hz波形，未发现滞后阶段的减少。然而，在MEF早期，细菌素的产量显着增加60 Hz高频处理在这些观察的基础上，通过在生长的早期应用纯正弦MEF来加速发酵过程，而在60 Hz的谐波下以60 Hz的正弦电场应用正弦磁场时，细菌素的产生显着增加。增长。

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