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首页> 外文期刊>Biotechnology Progress >Characterization of Protein Capacity of Nanocation Exchanger Particles as Filling Material for Functional Magnetic Beads for Bioseparation Purposes
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Characterization of Protein Capacity of Nanocation Exchanger Particles as Filling Material for Functional Magnetic Beads for Bioseparation Purposes

机译：纳米阳离子交换颗粒作为功能性磁珠用于生物分离的填充材料的蛋白质容量表征

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摘要

In this study we describe the synthesis and characterization of nanocation exchanger particles (NCEX) as the functional filling material for magnetic beads. Polystyrene NCEX particles were synthesized from styrene via a miniemulsion polymerization. The coupling of cation exchanger groups was done with chlorosulfuric acid after the polymerization reaction. The NCEX particles have an average diameter of 160—260 nm. Their ion exchange capacity amounts up to 4.58 mval/g. In an adsorption experiment it was possible to adsorb 192 mg lysozyme/g NCEX. Depending on the equilibrium concentration of lysozyme in the bulk solution 70—85% of the attached protein was desorbed. NCEX particles were used to produce magnetic beads with cation exchanger properties. Therefore an innovative production process for the synthesis of magnetic beads from different single components was used. The produced magnetic beads contained 40 wt % NCEX material and showed an ion exchanger capacity of 2 mequiv/g. It was possible to adsorb 75 mg lysozyme/g magnetic beads with a maximum recovery rate of 95%.
机译：在这项研究中，我们描述了纳米阳离子交换颗粒（NCEX）的合成和表征，作为磁性珠粒的功能性填充材料。聚苯乙烯NCEX颗粒是通过细乳液聚合从苯乙烯合成的。聚合反应后，阳离子交换基团的偶联用氯硫酸进行。 NCEX粒子的平均直径为160-260 nm。它们的离子交换容量高达4.58 mval / g。在吸附实验中，有可能吸附192 mg溶菌酶/ g NCEX。根据整体溶液中溶菌酶的平衡浓度，70-85％的附着蛋白被解吸。 NCEX颗粒用于生产具有阳离子交换剂性能的磁珠。因此，使用了创新的生产方法，用于从不同的单一组分合成磁珠。所产生的磁珠包含40重量％的NCEX材料，并且显示出2mequiv / g的离子交换容量。可以吸附75 mg溶菌酶/ g磁珠，最大回收率为95％。

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