...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of insect biotechnology and sericology >Optimization of an Enzyme-Linked Immunosorbent Assay for Ecdysteroids
【24h】

Optimization of an Enzyme-Linked Immunosorbent Assay for Ecdysteroids

机译：优化Ecdysteroids的酶联免疫吸附测定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The practical conditions of an enzyme-linked immunosorbent assay (ELISA) for ecdysteroids were optimized. A complex of ecdysone and horseradish peroxidase (HRP) was prepared as an enzyme tracer, and a competition between the tracer and free ecdysteroids was employed for the ELISA system, The binding of rabbit antise-rum against 20-hydroxyecdysone (20E) was better with the direct method than indirect method that used anti-rabbit IgG. Optimal blocking using 1% casein before the competition assay had lower background compared to no blocking or blocking using 1% BSA. The optimized method showed a determination range of 0.06 - 6 ng equivalent of 20E. The system made it possible to determine ecdysteroids in both in vitro and in vivo samples. Changes in ecdysteroid liter in hemolymph of Agrius convolvuli between 4th molting and eclosion determined by the ELISA optimized in this study was similar to that reported concentrations in Manduca sexta.
机译：优化了EcdeSteroids的酶联免疫吸附试验（ELISA）的实际条件。 制备了一种群体的蜕皮和辣根过氧化物酶（HRP）作为酶示踪剂，并且在elisa系统中使用示踪剂和游离蜕皮素之间的竞争，兔抗ris-rum对20-羟基粥粥（20e）的结合更好 直接方法比使用抗兔IgG的间接方法。 与使用1％BSA的无阻塞或阻断相比，使用1％酪蛋白的最佳阻断具有较低的背景。 优化方法显示了0.06-6 ng等效于20e的确定范围。 该系统使得可以在体外和体内样品中确定EcdeSteroids。 在本研究优化的ELISA测定的第4次蜕皮升温中Ecdysteroid升血淋升的变化与Manduca Sexta的浓度相似。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号