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首页> 外文期刊>Journal of insect biotechnology and sericology >Comparative Analyses of Deletion Mutations and the Pathological Effects of Nucleopolyhedroviruses Isolated from Saturniid Wild Silkworms
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Comparative Analyses of Deletion Mutations and the Pathological Effects of Nucleopolyhedroviruses Isolated from Saturniid Wild Silkworms

机译：缺失突变的比较分析及岩浆野生蚕中核多肽病毒的病理学作用

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摘要

Nucleopolyhedroviruses (NPVs) of saturniid wild silkworms are important in both insect pathology and biotechnology, because they cause severe damage to silk production in Asian countries and are also utilized as expression vectors for the production of valuable proteins in wild silkworms with larger body sizes than the domesticated silkworm, Bombyx mori. In this study, partial genomic DNA sequences of the Antheraea yamamai NPV (AnyaNPV) were determined and compared with the corresponding sequences ofthe A. pernyi NPV (AnpeNPV) and Samia ricini NPV (SariNPV). Their sequences were highly homologous, suggesting that they might be variants derived from a common ancestral NPV. They also shared large deletions in the coding region of the ecdys-teroid UDP-glucosyltransferase gene (egt), although a clone of SariNPV possessed a full-length egt. The 4th instar larvae of S. ricini infected with egt-deleted NPVs died earlier than those infected with a cloned SariNPV carrying the full-length egt, suggesting that the egt deletions reduce production of polyhedral occlusion bodies (POBs) in the infected insects. In addition, SariNPV possessed large deletions in the contiguous cathepsin and chitinase gene region (v-cath~chiA), and its infection to S. ricini larvae did not cause obvious liquefaction of the larval bodies as well as burst release of POBs, suggesting that the v-cath~chiA deletions reduce horizontal transmission in the insect population. These results seem to indicate that the deletions detected in the egt and/or v-cath~chiA regions of the saturniid NPVs may not be significantly disadvantageous and have some good reasons, at least, in their infection cycles under the rearing conditions of their hosts.
机译：Saturniid野生蚕的核多肽（NPV）在昆虫病理学和生物技术方面都很重要，因为它们对亚洲国家的丝绸生产造成严重损害，并且也被用作在野生蚕中生产有价值的蛋白质的表达载体，而且驯养的桑蚕，Bombyx Mori。在该研究中，测定了Antheraea yamamai NPV（AnyanPV）的部分基因组DNA序列并与A.Pernyi NPV（AnpenPV）和Samia Ricini NPV（SarinPV）的相应序列进行比较。它们的序列是高度同源的，表明它们可能是衍生自共同的祖先NPV的变体。它们还在Ecdys-Teroid UDP-葡糖基转移酶基因（EGT）的编码区域中分享了大量缺失，尽管SarinPV的克隆具有全长EGT。 S.RICINI的第4次INSTAR RICINE感染EGT缺失的NPVs早于感染携带全长EGT的克隆SarinPV的NPV，这表明EGT缺失减少了感染昆虫中的多面体闭塞体（POB）的产生。此外，Sarinpv在连续的组织蛋白酶和逐胰酶基因区（V-Cath〜Chia）中具有大量缺失，并且其对幼虫的感染没有引起幼虫体的明显液化，以及豆腐的爆发释放，表明这一点V-Cath〜Chia缺失减少了昆虫人群水平传输。这些结果似乎表明，在EGT和/或V-Cath〜Chia区域中检测到的燕雀NPVs的缺失可能不会显着不利，并且至少在其主持人的饲养条件下的感染周期中具有一些好的原因。

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