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首页> 外文期刊>Journal of insect biotechnology and sericology >A method for cryopreservation of ovaries of the ladybird beetle,Harmonia axyridis
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A method for cryopreservation of ovaries of the ladybird beetle,Harmonia axyridis

机译：一种瓢虫甲虫卵巢的冷冻保存方法，Harmunia Axyridis

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The development of genome editing techniques has allowed the generation of tremendous genetic diversity, even in non-model insects. The major obstacle for maintenance of genetically diverse stocks in non-model insects is the need for constant rearingin the laboratory, which is labor-intensive and time-consuming. The maintenance of insect colonies in the laboratory is further complicated by risk factors such as disease contamination, human error, and genetic changes by natural mutations that can leadto the loss of desirable genotypes. To avoid these risk factors, cryopreservation is the most desirable option. Here, we present a method we developed for cryopreservation of ovary from the multicolored Asian ladybird beetle, Harmonia axyridis, adaptedfrom techniques for ovary cyropresevation in the silkworm, Bombyx mori. We used last (4th) instar beetle larvae as both donors and recipients for the cryopreserved ovaries. The best and average success rates of cryopreservation were 40% and 26%, respectively, based on the percentage of beetles receiving cryopreserved ovary transplants that subsequently laid viable eggs. This success rate is much higher than that reported for B. mori using last in-star larvae. The 26% average success rate is sufficient for the technique to be of practical use for maintaining genetically diverse lines of H. axyridis, and reduces the laborious tasks required for constant maintenance of numerous colonies. This is the first reported success in ovary cryopreservation for a small non-model insect. The techniques that we developed should also be useful for ovary cryopreservation in other small non-model insects.
机译：即使在非模型昆虫中，基因组编辑技术的发展也允许产生巨大的遗传多样性。在非模型昆虫中维护转基因股票的主要障碍是实验室持续后期的需要，这是劳动密集型和耗时的。在实验室中的昆虫菌落的维持是由于疾病污染，人体错误和遗传变化的危险因素，可以引起可引起所需基因型的丧失的危险因素。为避免这些风险因素，冷冻保存是最理想的选择。在这里，我们提出了一种用于从多彩多姿的亚洲瓢虫甲虫，Harmunia anyridis的卵巢冷冻​​保存的方法，适应了家蚕，Bombyx Mori的卵虫中卵巢筛选的技术。我们使用最后（第4）型甲壳虫幼虫作为冷冻保存卵巢的捐助者和接受者。基于接受随后铺设可行卵的甲虫的甲虫百分比，冷冻保存的最佳和平均成功率分别为40％和26％。这种成功率远高于使用最后一星幼虫的B. Mori的成功率。 26％的成功率足以使该技术具有实际用途，用于维持遗传多样化的H.Axyridis，并减少持续维持许多菌落所需的费力任务。这是第一次报道卵巢冷冻保存的成功，用于小型非模型昆虫。我们开发的技术也应该有助于其他小型非模型昆虫的卵巢冷冻​​保存。

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