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首页> 外文期刊>Journal of insect biotechnology and sericology >Genetic studies on the novel lethal mutant, light orange lethal, in the silkworm, Bombyx mori
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Genetic studies on the novel lethal mutant, light orange lethal, in the silkworm, Bombyx mori

机译：关于新型致命突变体，浅橙色致命的遗传研究，在蚕，Bombyx Mori

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Many lethal mutations of the silkworm egg have been reported. The lethal phenotype is exhibited at several stages including blastokinesis, head pigmentation, and body pigmentation. We recently isolated a novel lethal mutant, which the skin color of the mature embryo became a light orange and the embryo died before hatching. In this study, we performed morphological observation and genetic analysis of this lethal mutation. Phenotypic observation showed that the phenotype of this lethal mutant is quitesimilar to that of the sex-linked chocolate lethal (sen), chocolate (ch), chocolate 2 (ch-2), and maternal chocolate (cm) mutants. We first performed the crossing experiment, which revealed that this mutation is regulated by a single recessive gene, andis not linked to the sex chromosome on which the sch1 mutation is located. We next performed the pre-linkage test with this lethal mutation and three other mutations, which revealed that an allelic relationship among these mutations could not be detected. Therefore, we determined that this is a novel lethal mutant, which we named light orange lethal (l-og). We next performed the linkage analysis of the l-og mutation using 20 F2 individuals and primer sets designed for each chromosome. The linkage analysis revealed that the l-og mutation is definitely located on chromosome 10. Furthermore, the rough mapping using 119 F2 individuals and 7 primer sets designed for chromosome 10 revealed that the /-og-linked region was narrowed down to approximately 3.7 Mb long on chromosome 10.
机译：已经报道了蚕卵的许多致命突变。致命表型在包括囊肿，头部色素沉着和体色素沉着的几个阶段。我们最近孤立一种新型致命突变体，成熟胚胎的肤色成为浅橙色，胚胎在孵化前死亡。在这项研究中，我们对这种致命突变进行了形态学观察和遗传分析。表型观察表明，这种致死的突变体的表型与性关联巧克力致死（SEN），巧克力（CH），巧克力2（CH-2）和母巧克力（CM）突变体的耻骨型。我们首先进行了交叉实验，表明这种突变由单个隐性基因调节，而没有与Sch1突变所在的性染色体相关的。我们接下来与这种致命突变和三种其他突变进行了预先结合试验，揭示了不能检测到这些突变之间的等位基关系。因此，我们确定这是一种新的致命突变体，我们命名为浅橙色致命（L-OG）。接下来我们使用20 F2个体和为每种染色体设计的引物组进行L-OG突变的连杆分析。连杆分析显示，L-OG突变绝对位于染色体10上。此外，使用119 F2个体和为染色体10设计的7个引物组的粗糙映射显示/ -Og连接区域缩小到约3.7mb长染色体10。

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