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首页> 外文期刊>Journal of industrial and engineering chemistry >High Performance Detection of Alzheimer's Disease Biomarkers Based on Localized Surface Plasmon Resonance
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High Performance Detection of Alzheimer's Disease Biomarkers Based on Localized Surface Plasmon Resonance

机译：基于局部表面等离子体共振的阿尔茨海默病生物标志物高性能检测

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In this study, we fabricated sensors to detect Alzheimer's disease (AD) biomarkers - amyloid beta (1-42) (A beta(1-)(42)) based on localized surface plasmon resonance (LSPR). The sensors were consisted of ligand-exchanged gold nanoparticles (Au NPs) deposited on polyethylene terephthalate substrate using Langmuir-Blodgett (LB) technique. Monoclonal antibodies (anti-A beta(1-)(42)) were then immobilized as a conjugate form with biotin onto the LB films of ligand-exchanged Au NPs by the aid of streptavidin. The attachment of the biomarkers to the antibodies immobilized on the LB films was detected by measuring absorbance change of plasmonic response peak. The sensor structure was optimized by comparing the results for the Au NPs LB films with different size and film thickness. The optimized sensor was used to detect biomarker at different concentrations in the buffer solution and a diluted cerebrospinal fluid (CSF) solution. As results, the sensor was able to detect even a trace amount of A beta(1-)(42) as small as 1 pg/ml from the CSF. This prototype sensor has a great potential because it shows several advantages of facile and inexpensive fabrication, and early detection of the AD. (C) 2020 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
机译：在这项研究中，我们制造了基于局部表面等离子体共振（LSPR）的阿尔茨海默病（Ad）生物标志物 - 淀粉样蛋白β（1-42）（β（1 - ）（42））。传感器由使用Langmuir-Blodgett（LB）技术沉积在聚对苯二甲酸乙二醇酯基质上的配体交换的金纳米颗粒（Au NP）组成。然后将单克隆抗体（抗-β（1 - ）（42））固定为用链霉蛋白的帮助，用生物素与生物素的缀合物形式与Biotin相交的Au NPS的LB膜一起。通过测量等离子体反应峰的吸光度变化，检测生物标志物与固定在LB膜上固定的抗体的抗体。通过比较具有不同尺寸和膜厚度的Au NPS LB膜的结果来优化传感器结构。优化的传感器用于检测缓冲溶液中不同浓度的生物标志物和稀脑脊髓液（CSF）溶液。结果，传感器能够从CSF检测均匀的β（1 - ）（42）的痕量β（1 - ）（42）。该原型传感器具有很大的潜力，因为它显示了易于和廉价的制造的几个优点，以及广告的早期检测。 （c）2020朝鲜工业与工程化学学会。 elsevier b.v出版。保留所有权利。

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