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首页> 外文期刊>Journal of industrial and engineering chemistry >Highly photostable rylene-encapsulated polymeric nanoparticles for fluorescent labeling in biological system
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Highly photostable rylene-encapsulated polymeric nanoparticles for fluorescent labeling in biological system

机译：用于荧光标记的高度光稳定的亚里烯包封的聚合物纳米颗粒在生物系统中的荧光标记

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摘要

Rylene dyes exhibit superior photophysical properties including outstanding chemical and photochemical stabilities as well as high fluorescence quantum yields (FQYs). However, the rylene chromophores have some limitations, such as high hydrophobicity and low solubility in aqueous solution, as a fluorescent tag for biological system. Herein, we present a general method to load the rylene dyes into triblock copolymer nanoparticles (NPs) and stabilize the core. The rylene dyes in confined hydrophobic core fairly preserve their FQYs in aqueous environment. Lumogen probe nanoparticles (PNPs) were prepared with four different commercial rylenes (Lumogen Violet, Yellow, Orange and Red) in the spectral region of visible and infrared (400-750 nm) and their photophysical properties were characterized. They exhibited enhanced photostability compared to commercial fluorescent dyes. Also the internalization of Lumogen PNPs in HeLa cell was confirmed by cell uptake test. These results indicate the Lumogen PNPs can be widely applicable in fluorescence detection and imaging such as flow cytometry and fluorescence microscopy. (C) 2019 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
机译：亚丁烯染料表现出优异的光学性质，包括优异的化学和光化学稳定性以及高荧光量子产率（FQYS）。然而，亚亚丁烯发色团具有一些限制，例如在水溶液中具有高疏水性和低溶解度，作为生物系统的荧光标签。在此，我们提出了一种将亚亚乙烯染料将亚甲酯染料装入三嵌段共聚物纳米颗粒（NPS）中的一般方法并稳定核心。狭窄的疏水核心中的亚丁烯染料在水环境中保持了它们的FQYS。在可见光和红外线（400-750nm）的光谱区域中，用四种不同的商业亚丁烯（Lumogen紫，黄色，橙色和红色）制备岩性探针纳米颗粒（PNP），其表征它们的光性学性质。与商业荧光染料相比，它们表现出增强的光稳定性。此外，通过细胞摄取试验证实了HeLa细胞中储层PNP的内化。这些结果表明储层PNP可广泛应用于荧光检测和成像，例如流式细胞术和荧光显微镜。 （c）2019年韩国工程化学学会。 elsevier b.v出版。保留所有权利。

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